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United States Department of Agriculture

Agricultural Research Service

Research Project: Innovations that Improve the Efficiency and Effectiveness of Managing and Preserving Ex Situ Plant Germplasm Collections

Location: Plant Germplasm Preservation Research Unit

Title: Cryopreservation of vitis shoot tips from diverse species

Authors
item Shepherd, Ashley -
item Bonnart, Remi
item Volk, Gayle

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: August 12, 2013
Publication Date: August 12, 2013
Citation: Shepherd, A., Bonnart, R.M., Volk, G.M. 2013. Cryopreservation of vitis shoot tips from diverse species. Meeting Abstract. Second International Symposium on Plant Cryopreservation, p.103.

Technical Abstract: Cryopreservation, long term storage of materials in liquid nitrogen (LN), is an effective method for backing-up national fruit collections. When efficient methods are available, cryopreservation has become a routine addition to the genebanking process and provides off-site security for collections. We have identified methods that successfully cryopreserve Vitis shoot tips from diverse species. Traditionally, plants from field collections have been introduced into tissue culture for a multiplication step prior to shoot tip excision. This in vitro step created limitations on the amount of materials that could be processed. We, therefore, sought to eliminate the in vitro step by using shoot tips excised from greenhouse-grown materials. Our efforts focus on improving the efficiency of Vitis cryopreservation by optimizing the selection of shoot tip source material, cryoprotectant exposure times, desiccation timecourses, and freezing rates to ensure high survival post liquid-nitrogen exposure.

Last Modified: 11/27/2014
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