Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Plant and Microbial Genetic Resource Preservation and Quality Assessment

Location: Plant And Animal Genetic Resources Preservation Research Unit

Title: Droplet vitrification technique for cryopreservation of different pineapple (Ananas comosus L. Merrill) accessions

Authors
item Souza, Fernanda -
item Kaya, Ergun -
item Oliveira, V -
item Skogerboe, Dianne
item Vieira, Livia -
item Alves, Alfredo -
item Matsumoto Brower, Tracie
item Jenderek, Maria

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: July 16, 2013
Publication Date: August 11, 2013
Citation: Souza, F.V., Kaya, E., Oliveira, V., Skogerboe, D.M., Vieira, L., Alves, A.A., Matsumoto Brower, T.K., Jenderek, M.M. 2013. Droplet vitrification technique for cryopreservation of different pineapple (Ananas comosus L. Merrill) accessions. Meeting Abstract. 2nd International Symposium on Plant Cryopreservation, Fort Collins, CO, Aug 11-14, 2013. pp. 66.

Technical Abstract: Germplasm conservation of pineapple is crucial to secure the genetic variability of the genus for breeding programs and supporting new research. Long-term conservation is done through cryopreservation, by storing cells or tissues at ultra-low temperature in liquid nitrogen (LN; -196°C) or in the LN vapor phase (-150°C). Droplet-vitrification, a combination of droplet-freezing and solution-based vitrification were used to establish a protocol for cryopreservation of pineapple genetic resources. Excised shoot tips (1 mm) with one primordial leaf from three different accessions (Perola, an ornamental hybrid and MD2) of pineapple were pre-cultured for 48 h on solid MS medium containing 0.3 M sucrose. Three exposure times of PVS2 (30, 45 and 60 min.) were tested. The results showed a high survival rates to all three genotypes. The best time of PVS2 exposure varied among the genotypes and the highest number of shoots surviving the LN exposure was observed for the MD2 shoots exposed to PVS2 for 45 min (60%). Plants regenerated from LN stored shoots showed a typical growth for pineapple cultured in vitro and no morphological changes were observed.

Last Modified: 7/28/2014
Footer Content Back to Top of Page