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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Cool Season Food Legumes

Location: Grain Legume Genetics Physiology Research

Title: Inheritance and linkage map positions of genes conferring agromorphological traits in Lens culinaris Medik

Authors
item Saha, Gopesh -
item Sarker, Ashutosh -
item Chen, Yung Chun
item Vandemark, George
item Muehlbauer, Fred -

Submitted to: International Journal of Agronomy
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 19, 2013
Publication Date: June 30, 2013
Citation: Saha, G., Sarker, A., Chen, Y., Vandemark, G.J., Muehlbauer, F. 2013. Inheritance and linkage map positions of genes conferring agromorphological traits in Lens culinaris Medik. International Journal of Agronomy. vol. 2013, Article ID 618926, 9 pages. doi:10.1155/2013/618926.

Interpretive Summary: Lentil is a staple crop in many parts of the world and an important rotational crop in cereal-based production systems. However, the inheritance and genetic control of many agro-morphological traits of lentil are not known. This study was initiated to study the genetics and identify map positions of some important agro-morphological traits including days to flowering, plant height, seed diameter, 100 seed weight, cotyledon color, and growth habit of lentil. Recombinant inbred line population developed from a cross (ILL-5888'×'ILL-6002) was used in this study, Three quantitative trait loci were detected for days to 50% flowering. Although epistatic effects accounted for most of the variation in plant height, main effect of one QTL accounted for 15.3% variation in plant height. Three QTLs were detected seed diameter, and one of them accounted for 32.6% of the variation. Five QTLs were identified for 100 seed weight. A single dominant gene controls prostrate over erect growth habit, and a single dominant gene control red over yellow cotyledon color. The QTL information generated in this study will very useful in assisting selection of breeding lines for early maturity, upright growth habit, and improved seed quality.

Technical Abstract: Agromorphological traits have immense importance in breeding lentils for higher yield and stability. We studied the genetics and identified map positions of some important agro-morphological traits including days to 50% flowering, plant height, seed diameter, 100 seed weight, cotyledon color, and growth habit in Lens culinaris. Earlier developed RILs for stemphylium blight resistance (ILL-5888'×'ILL-6002), contrasted for those agro-morphological traits, were used in our study. Three QTLs for days to 50% flowering were detected with additive and epistatic effects. One QTL for days to 50% flowering, QLG483 (QTL at linkage group 4 at 83'cM position), accounted for an estimated 20.2% of the variation, while QLG124'×'QLG1352 and QLG484'×'QLG138 accounted for 15.6% and 24.2% of the variation, respectively. Epistatic effects accounted for most of the variation in plant height, but the main effect of one QTL, QLG84, accounted for 15.3%. For seed diameter, three QTLs were detected, and one QTL, QLG482, accounted for 32.6% of the variation. For 100 seed weight, five QTLs were identified with significant additive effects and four with significant interaction effects. The main effect of one QTL, QLG482, also accounted for 17.5% of the variation in seed diameter. QLG482-83 which appears to affect days to 50% flowering, seed diameter, and 100 seed weight is flanked by RAPD markers, UBC 34 and UBC1. Growth habit and cotyledon color are controlled by single genes with prostrate dominant to erect and red cotyledon dominant to yellow. The QTL information presented here will assist in the selection of breeding lines for early maturity, upright growth habit, and improved seed quality.

Last Modified: 12/21/2014
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