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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Analysis of Poultry-Associated Salmonella enterica to Identify and Characterize Properties and Markers Associated with Egg-Borne Transmission of Illness

Location: Egg Safety and Quality

Title: Presence of Salmonella Enteritidis and Salmonella Gallinarum in commercial laying hens diagnosed with Fowl Typhoid Disease in Colombia

Authors
item Pulido-Landinez, Martha -
item Sanchez-Ingunza, Roxana -
item Guard, Jean
item Pinheiro Do Nascimen, To. V -

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 5, 2013
Publication Date: September 16, 2013
Citation: Pulido-Landinez, M., Sanchez-Ingunza, R., Guard, J.Y., Pinheiro Do Nascimen, T. 2013. Presence of Salmonella Enteritidis and Salmonella Gallinarum in commercial laying hens diagnosed with Fowl Typhoid Disease in Colombia. Avian Diseases. p. 165-170.

Technical Abstract: : A severe outbreak of salmonellosis in commercial brown table egg layers first occurred in Colombia in 2006. From 2008 to 2012, 35 samples collected from commercial layers farms in the states of Cundinamarca, Santander, Bolivar and San Andres, were positive to Salmonella enterica. Salmonella (S) was isolated from liver and spleen (71.42%), pools of organs (liver, spleen and ovarian follicles), (25.71%) and drag swabs (2.85%). Serotype was assigned using single nucleotide polymorphisms (SNPs) or DNA microarray hybridization. Sixteen strains of S. Enteritidis and 13 of S. Gallinarum were identified. Seven strains yielded 3 unique sequences and they were designated as UN0038, UN0052 and UN0054 by intergenic sequence ribotyping. These strains were later identified as serotypes Isangi, Braenderup and Yoruba, respectively, by DNA microarray hybridization. The discovery that a common human pathogen (S. Enteritidis) was co-isolated from farms with an avian pathogen (S. Gallinarum) in similar commercial brown layer hens and in different regions indicates that it is important to investigate the dynamics of Salmonella infection and determine the serotypes circulating within the same ecological niche.

Last Modified: 12/20/2014
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