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United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Hard Winter Wheat to Biotic and Abiotic Stresses

Location: Hard Winter Wheat Genetics Research Unit

Title: Differentially expressed proteins associated with Fusarium head blight resistance in wheat

Authors
item Zhang, Xianghui -
item FU, JIANMING
item Hiromasa, Yasuaki -
item Pan, Hongyu -
item BAI, GUIHUA

Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 29, 2013
Publication Date: December 20, 2013
Repository URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0082079#pone-0082079-g003
Citation: Zhang, X., Fu, J., Hiromasa, Y., Pan, H., Bai, G. 2013. Differentially expressed proteins associated with Fusarium head blight resistance in wheat. PLoS One. 8(12):e82079.

Interpretive Summary: Fusarium head blight (FHB) substantially reduces wheat grain yield and quality worldwide. Proteins in resistant plants play important roles in defense against the fungal infection. This study characterized differentially expressed proteins between near-isogenic lines (NILs) with and without FHB resistance gene Fhb1 to identify proteins regulating FHB resistance. Eight proteins were induced in pathogen-inoculated resistant NIL, but not in the NIL inoculated with water. Nine proteins were induced in the pathogen-inoculated resistant NIL but not in the susceptible NIL and these proteins mainly have functions of defending fungal penetration, photosynthesis, energy metabolism, and detoxification. Coordinated expression of these proteins might result in FHB resistance in the resistant NIL. The results provide insight into the pathway of Fhb1-mediated FHB resistance.

Technical Abstract: Fusarium head blight (FHB), mainly caused by Fusarium graminearum, substantially reduces wheat grain yield and quality worldwide. Proteins play important roles in defense against the fungal infection. This study characterized differentially expressed proteins between near-isogenic lines (NILs) contrasting in alleles of Fhb1, a major FHB resistance gene in wheat, to identify proteins underlining FHB resistance of Fhb1. The two-dimensional protein profiles were compared between the Fusarium-inoculated spikes of the two NILs collected 72 h after inoculation. The protein profiles of mock- and Fusarium-inoculated Fhb1+NIL were also compared to identify pathogen-responsive proteins. Eight proteins were either induced or upregulated in inoculated Fhb1+NIL when compared with mock-inoculated Fhb1+NIL; nine proteins were either induced or upregulated in the Fusarium-inoculated Fhb1+NIL when compared with Fusarium-inoculated Fhb1-NIL. Proteins that were differentially expressed in the Fhb1+NIL, not in the Fhb1-NIL, after Fusarium inoculation included wheat proteins for defending fungal penetration, photosynthesis, energy metabolism, and detoxification. Coordinated expression of the identified proteins resulted in FHB resistance in Fhb1+NIL. The results provide insight into the pathway of Fhb1-mediated FHB resistance.

Last Modified: 9/10/2014
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