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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMICS AND ENGINEERING OF STRESS TOLERANT MICROBES FOR LOWER COST PRODUCTION OF ETHANOL FROM LIGNOCELLULOSE

Location: Bioenergy Research Unit

Title: Engineering industrial yeast for renewable advanced biofuels applications

Author
item Liu, Zonglin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 28, 2013
Publication Date: N/A

Technical Abstract: The industrial yeast Saccharomyces cerevisiae is a candidate for the next-generation biocatalyst development due to its unique genomic background and robust performance in fermentation-based production. In order to meet challenges of renewable and sustainable advanced biofuels conversion including cellulosic ethanol production, it is necessary to engineer the yeast with enriched capabilities to overcome toxic chemical compounds liberated from lignocellulose-biomass pretreatment and to efficiently utilize pentose and hexose sugars harbored in the biomass. Using a systems biology approach including evolutionary engineering and genetic engineering, we developed new genotypes of industrial yeast strains that are able to utilize xylose and glucose simultaneously and in situ detoxify major biomass pretreatment inhibitors such as furfural and 5-hydroxymethylfurfural while producing ethanol. This presentation will outline major technical difficulties and discuss successful strategies for this advanced development. Challenges in new strain development toward industrial applications will also be discussed.

Last Modified: 11/26/2014
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