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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Tools for Improved Crop Biotechnology

Location: Crop Improvement & Utilization Research

Title: Novel gene expression tools for rice biotechnology

Authors
item THILMONY, ROGER
item GUTTMAN, MARA
item Cook, Meridith

Submitted to: Proceedings International Rice Genetic Symposium
Publication Type: Abstract Only
Publication Acceptance Date: July 9, 2013
Publication Date: N/A

Technical Abstract: Biotechnology is an effective and important method of improving both quality and agronomic traits in rice. We are developing novel molecular tools for genetic engineering, with a focus on developing novel transgene expression control elements (i.e. promoters) for rice. A suite of monocot grass promoters that confer distinct patterns of organ-specific or stress-responsive expression in transgenic plants is being created. Candidate genes with distinct potentially useful patterns of expression were identified and the corresponding upstream promoter sequences were fused to a reporter gene and transformed into rice, wheat, Brachypodium distachyon and/or Arabidopsis plants. The characterization of root-specific, pollen-specific and green tissue-specific promoters will be presented. We are also developing the capability of genetically engineering rice using only rice-derived sequences. Research utilizing this novel strategy to generate ‘intragenic’ rice plants that carry introduced traits comprised of only native sequences will be presented. These new biotech tools will enable spatially-defined transgene expression in cereal crops and can potentially facilitate the development of novel biotech rice.

Last Modified: 7/25/2014
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