Title: Registration of purified accessions for the U.S. peanut mini core germplasm collection Authors
Submitted to: Journal of Plant Registrations
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 5, 2013
Publication Date: September 27, 2013
Citation: Chen, C., Barkley, N.L., Wang, M.L., Holbrook Jr, C.C., Dang, P.M. 2013. Registration of purified accessions for the U.S. peanut mini core germplasm collection. Journal of Plant Registrations. 8(1):77-85. Interpretive Summary: The peanut mini core is a genetically diverse subset derived from the entire peanut germplasm collection with minimal genetic redundancy. This collection has been evaluated (morphologically, biochemically, and via DNA fingerprinting) due to its small manageable size (112 lines). Many of the lines in this subset were highly variable since they were originally collected as wild plant populations. The lines were purified by growing them in the field to produce seed and removing any off types for several years. Producing pure lines are critical in order to effectively carry out genomic studies. These purified lines are now available for peanut researchers to request from the USDA-ARS PGRCU in Griffin, GA.
Technical Abstract: Many accessions of the USDA peanut (Arachis hypogaea L.) germplasm collection are heterogeneous. Advances in genomics technology have highlighted the need for collections of homogeneous accessions. The objectives of this research were to purify accessions of the USDA mini-core collection and to characterize this collection for morphological traits. Twenty seeds of one hundred and four accessions of the peanut mini-core collection were planted at Dawson, GA in 2008 under irrigated conditions. The seeds from five phonotypical uniform plants were harvested, bulked, and planted in Headland, AL in 2009 to continue the purification process. In 2010, homogenous seeds of each accession were planted in Headland, AL to increase seed counts for chemical analysis and genotyping. Besides morphological characters and seed chemical composition, the accessions were evaluated for spotted wilt resistance caused by a tomato spotted wilt virus (TSWV) and early leaf spot resistance caused by Cercospora arachidicola (Hori) which is a major disease that significantly impact peanut production in the southeast. The morphological and chemical data, along with images of pod and seed traits were entered in the Germplasm Resources Information Network (GRIN) and detailed information can be found at: http://www.ars-grin.gov/cgi-bin/npgs/html/. The results showed that after purification, these accessions not only preserved similar genetic variation in the collection, but also became more homogeneous compared to the original accessions. A small amount of seed of the purified accession can be obtained for research and breeding purposes through the National Plant Germplasm System (http://www.ars-grin.gov/npgs/orders.html).