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United States Department of Agriculture

Agricultural Research Service

Research Project: GENOMICS AND PROTEOMICS APPROACHES TO BROADENING RESISTANCE OF SOYBEAN TO PESTS AND PATHOGENS

Location: Soybean Genomics and Improvement

Title: Gene expression in leaves of susceptible Glycine max during infection with Phakopsora pachyrhizi using Next-Generation sequencing

Authors
item Tremblay, Arianne -
item Hosseini, Parsa -
item Alkharouf, Nadim -
item LI, SHUXIAN
item MATTHEWS, BENJAMIN

Submitted to: Sequencing
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 7, 2011
Publication Date: December 1, 2011
Repository URL: http://doi:10.1155/2011/827250
Citation: Tremblay, A., Hosseini, P., Alkharouf, N.W., Li, S., Matthews, B.F. 2011. Gene expression in leaves of susceptible Glycine max during infection with Phakopsora pachyrhizi using Next-Generation sequencing. Sequencing. doi:10.1155/2011/827250.

Interpretive Summary: Soybean rust is a fungal disease that attacks soybean. We analyzed the expression pattern of soybean genes to understand molecular events occurring in soybean following the infection. We identified genes that are highly expressed during the interaction of soybean leaves with the fungus. Some of these genes may be candidates for genetic engineering of soybean to make soybean resistant to the fungus. This information is of interest to scientists and companies working to improve resistance of soybean to SR.

Technical Abstract: Soybean rust is caused by Phakopsora pachyrhizi and is a fungal disease that attacks soybean. We used an mRNA-Seq strategy to analyze the expression pattern of soybean genes and better understand molecular events occurring in soybean following the infection. cDNA libraries were constructed from RNA isolated from whole infected soybean leaves 10 days after inoculation with P. pachyrhizi and sequenced using an Illumina platform to identify soybean genes that are affected by pathogen growth. Forty-two percent of the genes were downregulated including genes encoding proteins involved in amino acid metabolism, carbohydrate metabolism, and transport facilitation. We identified genes that are highly expressed that may be candidates to make soybean resistant to the fungus. This information is of interest to scientists and companies working to improve resistance of soybean to soybean rust.

Last Modified: 9/10/2014
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