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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Genetics, Genomics, and Phylogenetics of Foodborne Zoonotic Parasites Affecting Food Safety and Public Health

Location: Animal Parasitic Diseases

Title: Unique antigenic gene expression at different developmental stages of Trichinella pseudospiralis

Authors
item Wu, X -
item Liu, X -
item Wang, W -
item Blaga, R -
item Fu, B -
item Liu, P -
item Bai, X -
item Wang, Z -
item Rosenthal, Benjamin
item Shi, H -
item Sandrine, L -
item Vallee, I -
item Boireau, P -
item Wang, F -
item Zhou, X -
item Zhao, Y -
item Liu, M -

Submitted to: Veterinary Parasitology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 29, 2013
Publication Date: May 20, 2013
Citation: Wu, X.P., Liu, X.L., Wang, W.L., Blaga, R., Fu, B.Q., Liu, P., Bai, X., Wang, Z.J., Rosenthal, B.M., Shi, H.N., Sandrine, L., Vallee, I., Boireau, P., Wang, F., Zhou, X.N., Zhao, Y., Liu, M.Y. 2013. Unique antigenic gene expression at different developmental stages of Trichinella pseudospiralis. Veterinary Parasitology. 194:198-201.

Interpretive Summary: For foodborne parasites in the genus Trichinella, it was once thought that a capsule surrounding the infectious larvae was a defining characteristic. Here, a species lacking such a capsule (Trichinella pseudospiralis) was compared to Trichinella spiralis in terms of the major antigens expressed at various points in its development. Parasites of two species evidently utilize different molecules and mechanisms for larval capsule formation and host immunosuppression. Proteins of antigenic genes identified in this study may be also good candidates for diagnosis, treatment and vaccination for T. pseudospiralis infection, and also for the differential diagnosis of two species’ infections. Thus, these findings will be of interest to those seeking improved understanding of the biology of these parasites, as well as those developing diagnostic assays based on immune responses of human beings or our food animals.

Technical Abstract: Parasite-induced and parasite-regulated larval capsule formation and host immunosuppression are two major characteristics that are unique in Trichinella spp. infections, but the molecule(s) and mechanism(s) that mediate these processes remain largely unknown. Trichinella pseudospiralis and Trichinella spiralis are obviously different with these two characteristics. The comparative study of these two species, especially of their antigen expression at different developmental stages (the main molecules involved in the cross-talk or interaction between each parasite and its host), may help us better understand the parasite molecules and mechanisms involved. Here, we constructed cDNA libraries of T. pseudospiralis adults, newborn larvae and muscle larvae and screened them with pig anti-T. pseudospiralis serum collected 26, 32 and 60 days post-infection (p.i.). The most abundant antigens were thereby found to vary among life history stages of T. pseudospiralis. A pyroglutamy peptidase 1-like and 6-phosphogluconolactonase-like genes predominated in the Ad stage; as with T. spiralis, a serine protease (SS2-1-like gene) predominated in new born larvae. Muscle larvae expressed a proteasome activator complex subunit 3- like and a 21 kDa excretory/secretory protein-like genes. The study indicated that parasites of two species may utilize different molecules and mechanisms for larvae capsule formation and host immunosuppression during their infections. Proteins of antigenic genes identified in this study may be also good candidates for diagnosis, treatment and vaccination for T. pseudospiralis infection, and also for the differential diagnosis of two species’ infections. Keywords: T. pseudospiralis; Antigenic genes; Different developmental stages.

Last Modified: 10/31/2014
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