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ARS Home » Northeast Area » Geneva, New York » Grape Genetics Research Unit (GGRU) » Research » Publications at this Location » Publication #293520
Title: Grapevine powdery mildew resistance and susceptibility loci identified on a high-resolution SNP map

Author
item BARBA, PAOLA - Cornell University
item Cadle-Davidson, Lance
item HARRIMAN, JAMES - Cornell University
item GLAUBITZ, JEFF - Cornell University
item MAHANIL, SIRAPRAPA - Former ARS Employee
item HYMA, KATIE - Cornell University
item REISCH, BRUCE - Cornell University

Submitted to: Journal of Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/26/2013
Publication Date: 1/24/2014
Citation: Barba, P., Cadle Davidson, L.E., Harriman, J., Glaubitz, J., Mahanil, S., Hyma, K., Reisch, B. 2014. Grapevine powdery mildew resistance and susceptibility loci identified on a high-resolution SNP map. Journal of Theoretical and Applied Genetics. 127:73-84.

Interpretive Summary: We studied the inheritance of resistance to powdery mildew from wild Vitis rupestris cross-hybridized with cultivated V. vinifera ‘Chardonnay’. A new DNA sequencing technology was used to map 16,833 DNA sequences from these parents. This is the densest Vitis map published to-date and represents an improvement of at least 10 -fold over current standards for grapevine maps. Eleven of these 16,833 sequences on one chromosome predicted resistance from V. rupestris. Moreover, 10 sequences on a different chromosome from ‘Chardonnay’ were associated with susceptibility. This provides the first insight into the genetics of susceptibility from V. vinifera and can be used to improve powdery mildew resistance by selecting against their presence among breeding selections. This work improves our understanding of the nature of resistance in V. rupestris and ‘Chardonnay’ while developing high-resolution DNA tools to advance grapevine genomics and breeding.

Technical Abstract: Improved efficacy and durability of powdery mildew resistance requires knowledge of the genetics of resistance and susceptibility and high resolution maps to facilitate combining of multiple resistance genes with desirable traits. In the current study, we studied the inheritance of resistance and susceptibility of the wild Vitis rupestris, genotype B38, and cultivated V. vinifera ‘Chardonnay’ against grape powdery mildew (Erysiphe necator) using a F1 population obtained from this cross. Molecular markers were identified using Genotyping-by-Sequencing (GBS) to obtain a map of 16,833 Single Nucleotide Polymorphisms (SNPs) based on alignment to the grapevine reference genome sequence. With an average density of 36 SNP/Mbp and uniform coverage of the genome, it is the densest Vitis map published to-date and represents an improvement of at least 10 -fold over current standards for grapevine linkage maps. Genome wide association study and linkage disequilibrium analysis allowed the identification of 11 SNPs associated with one resistance locus from V. rupestris B38, which can be used to introgress resistance into cultivated grapevine varieties. Moreover, identification of 10 SNPs from ‘Chardonnay’ linked with one locus of susceptibility provides the first insight into the genetics of susceptibility from V. vinifera and can be used to improve powdery mildew resistance by selecting against their presence among breeding selections. This work improves our understanding of the nature of resistance in V. rupestris B38 and ‘Chardonnay’, while developing relevant tools to advance grapevine genomics and breeding.