Title: In vitro hemostatic, hydrogen peroxide production and elastase sequestration properties of nonwoven ultra clean greige cotton dressing Authors
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 5, 2013
Publication Date: N/A
Technical Abstract: Nonwoven UltraCleanTM Cotton (highly cleaned and hydroentangled, greige cotton) retains the native wax and pectin content (~2%) of the cotton fiber traditionally removed from scoured and bleached cotton gauze, yet potentially affording wound healing properties. In vitro thromboelastography, hydrogen peroxide generation and protease uptake assays were employed to compare a dressing prototype of Hydroentangled, UltraClean Cotton (HE-UCC) with scoured and bleached cotton gauze (SB-CG), and occlusive dressing. Thromboelastography (TEG) was used to measure coagulation of bovine blood in the presence of HE-UCC compared with SB-CG by monitoring enzymatic clotting reaction time (R), rate of fibrin formation (a), fibrinogen-mediated clot formation (K) and strength of clot formation (MA). The TEG analysis revealed coagulation was affected as follows; R (min.) = 4.1 +1.0, K (min) = 3.2 +0.6, a (deg.) = 45.2 +6.3, MA (mm) = 62.5 +3.3.; whereas SB-CG performance was; R (min.) = 9.6 +1.6, K (min) = 6.6 +2.2, a (deg.) = 32.8 +7.5, MA (mm) = 67.7 +3.9. Hence HE-UCC significantly accelerated clotting reaction time and rate of fibrin formation over SB-CG, with clot strength being similar. Pectin-based release of hydrogen peroxide enhances the granulation phase. The hydrogen peroxide production of HE-UCC was compared with pectin and a commercial pectin-containing dressing using fluorescent scopoletin/horseradish peroxidase. Over a 72 hour time course at a dose of 10 mg/mL, HE-UCC produced comparable levels of hydrogen peroxide (10-6) to 2.5% pectin and 0.1% of the pectin-containing occlusive dressing. Human neutrophil elastase sequestration (HNE) by HE-UCC was compared with SB-CG. Cotton dressing levels of 50mg/mL (mimicked wound fluid) have sufficient sites on HE-UCC and SB-GC to bind >90% of available HNE at chronic wound concentrations (50 mU HNE). In vitro assessment of HE-UCC reveals promising findings on its potential to initiate clotting, low level hydrogen peroxide production, and protease uptake.