Location: Reproduction Research
Title: Application of RNA-seq transcriptomic analysis to reproductive physiology of the pig: Insights into differential trophoblast function within the late gestation porcine placenta Authors
Submitted to: Pig Reproduction National Conference Proceedings
Publication Type: Review Article
Publication Acceptance Date: March 22, 2013
Publication Date: June 9, 2013
Citation: McNeel, A.K., Chen, C.T., Schroeder, S.G., Sonstegard, T.S., Dawson, H.D., Vallet, J.L. 2013. Application of RNA-seq transcriptomic analysis to reproductive physiology of the pig: Insights into differential trophoblast function within the late gestation porcine placenta. In: Rodriquez-Martinez, H., Soede, N.M., Flowers, W.L., editors. Control of Pig Reproduction IX. Leicestershire, UK: Context Products Ltd. pp. 175-183. Technical Abstract: The pig possesses an epitheliochorial form of placentation and lacks the invasive characteristics of primate and rodent placentae. In the pig, the fetal-maternal interface (FMI) is composed of an epithelial cell bilayer, with both maternal and fetal contributions remaining intact. Beginning around day 45 of gestation, the fetal side of the bilayer is composed of two types of trophoblasts: short cuboidal and tall columnar. Little is known about their contribution to placental function. To understand their functions and what makes these cell types different from each other; we performed laser capture microdissection and RNA-seq to characterize their transcriptome. Our results indicate that trophoblast cells express a variety of genes that code for transporters for carbohydrates, amino acids, and long chain fatty acids and triglycerides.