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United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTIONS TO REDUCE FOODBORNE PATHOGENS IN SWINE AND CATTLE

Location: Food and Feed Safety Research

Title: Simultaneous determination of cyromazine and dicyclanil in animal edible tissues using UPLC-MS/MS

Authors
item Hou, Xiaolin -
item Zhou, Degang -
item Huai, Wenhui -
item Beier, Ross
item Sun, Yingjian -
item Lu, Yan -
item Wu, Guojuan -
item Sun, Zhiwen -
item Wu, Yongnin -

Submitted to: Food Additives & Contaminants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: December 17, 2012
Publication Date: March 8, 2013
Repository URL: http://handle.nal.usda.gov/10113/57296
Citation: Hou, X., Zhou, D., Huai, W., Beier, R.C., Sun, Y., Lu, Y., Wu, G., Sun, Z., Wu, Y. 2013. Simultaneous determination of cyromazine and dicyclanil in animal edible tissues using UPLC-MS/MS. Food Additives & Contaminants: Part A. 30:660-665.

Interpretive Summary: Cyromazine and dicyclanil are insect growth regulators used in veterinary medicine for the prevention of myiasis in sheep, a disease resulting from the infestation of tissue by fly larvae. Residues of cyromazine and dicyclanil in food animal tissue may be a food safety issue due to their toxicity. This paper demonstrates the development of an innovative mass spectrometry method, an ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method, for the determination of cyromazine and dicyclanil residues in animal tissues. The homogenized meat samples were extracted and deproteinized with 1% trichloracetic acid and defatted with hexane. Solid-phase MCX cartridges were used to further clean up the extracts and then cyromazine and dicyclanil were quantified using a matrix-matched calibration curve. A matrix-matched calibration curve is made from standard cyromazine and dicyclanil solutions diluted with extracts of the animal tissue being evaluated. This process can compensate for possible errors due to components in the tissue being evaluated. The mean recoveries of cyromazine and dicyclanil were between 62.0% and 99.2%. The relative standard deviations for the method were below 9.94%. The developed dicyclanil analysis method was rapid, sensitive, and reliable. The results demonstrated that the developed method can be used for the quantitative analysis of cyromazine and dicyclanil residues in animal tissues.

Technical Abstract: Cyromazine and dicyclanil are used as insect growth regulators. This article describes an easy and innovative simultaneous extraction for residues of cyromazine and dicyclanil in food of animal origin and the confirmation procedure using an ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry. The samples were extracted and deproteinized with 1% trichloracetic acid in water-acetonitrile. Samples were defatted using hexane based on the degree of matrix complication, cleaned up on MCX cartridges, and then quantified using a matrix-matched calibration curve. The mean recoveries were all between 62.0% and 99.2%. The RSDs were all below 9.94%. The present method was rapid, sensitive, and reliable and can be applied to the quantitative analysis of these residues in animal tissues.

Last Modified: 7/25/2014
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