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United States Department of Agriculture

Agricultural Research Service

Research Project: Insecticide Resistance Management and New Control Strategies for Pests of Corn, Cotton, Sorghum, Soybean, and Sweet Potato

Location: Southern Insect Management Research Unit

Title: Elevated metabolic dextoxification associated with multiple/cross resistance to defferent insecticide classes in tarnished plant bug

Authors
item Zhu, Yu Cheng
item Luttrell, Randall

Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: January 16, 2013
Publication Date: February 16, 2013
Citation: Zhu, Y., Luttrell, R.G. 2013. Elevated metabolic dextoxification associated with multiple/cross resistance to defferent insecticide classes in tarnished plant bug. National Cotton Council Beltwide Cotton Conference. Proceedings 2013 Beltwide Cotton Conference, National Cotton Council, January 7-10, 2013, San Antonio, TX. pp. 1152-1155. http://www.cotton.org/beltwide/proceedings/2005-2013/index.htm

Technical Abstract: Tarnished plant bugs (TPB, Lygus lineolaris) were collected from multiple locations in the Delta regions of Mississippi, Arkansas, and Louisiana (covering 200 miles in E-W and N-S directions), and were subjected to bioassays to acephate, imidacloprid, dicrotophos, thiamethoxam, and sulfoxaflor (representing four insecticide classes: pyrethroids, organophosphates, neonicotinoids, and sulfoximine). Results showed that survival rates increased by 2-fold from May to September. Field populations exhibited up to a 10-fold difference in their susceptibility, and survival rates ranged from 7 to 76%. Populations collected around cotton fields were usually less susceptible than populations collected around soybean and corn fields. Regression analysis indicated that populations with higher survival rates from organophosphate insecticide treatments also had higher survival rates from neonicotinoid insecticide treatments with moderate correlation (R2 was up to 0.63; P<0.001). Examination of detoxification enzymes, synergism with different inhibitors, and gene expression profiles consistently indicated that many TPB populations have developed certain levels of multiple/cross resistances to different insecticide classes. The resistance is mainly conferred by elevated esterase and P450 oxidase gene expressions, hence increased detoxification in resistant bugs. To slow down the resistance evolution, precautions must be taken to reduce selection pressure by properly rotating insecticides with different modes of action and avoiding use of insecticides that may induce common resistance mechanisms in target populations.

Last Modified: 7/22/2014
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