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United States Department of Agriculture

Agricultural Research Service

Research Project: Molecular Biology of Human Pathogens Associated with Food

Location: Produce Safety and Microbiology Research

Title: Evidence of norovirus (NV)in a major produce production region watershed and a low density microarray strategy for detecting NV(Abstract)

Authors
item Mandrell, Robert
item Tian, Peng
item Lee, Bertram
item Yang, David
item Bates, Anne
item Cooley, Michael
item Carychao, Diana
item Vinje, Jan -
item Quiñones, Beatriz

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 25, 2012
Publication Date: N/A

Technical Abstract: The presence of Norovirus (NV) in watersheds in the vicinity of raw produce production is a concern. This could occur due to leaking septic systems, improper RV waste removal, improperly treated recycled wastewater, or other unknown factors. We used a qRT-PCR method to test for NV in rinsates from Moore Swabs collected usually every 2 weeks from 26 sites between Oct-2011 to Jul-2012 (487 swabs tested); 122 (25%) were considered NV+. The NV+ samples ranged between 11-42% by site; 8, 69 and 45 samples were G1+ only, G2+ only and G1+/G2+. The highest incidence of NV was in samples collected in the absence of any precipitation 5 days preceding the day of collection. In addition, a low density DNA microarray has been constructed for rapid and cheap detection of NV. Preliminary studies of RNA reference samples of known NV genogroup revealed specific detection of NV. Future studies will focus on analysis of NoroCORE samples for validation of the low density DNA microarray and utility for identification of NV genogroups in watershed samples, collected in a major produce production region in California.

Last Modified: 10/20/2014
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