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United States Department of Agriculture

Agricultural Research Service

Research Project: COTTON GENOMICS AND GENETIC ANALYSIS Title: De novo SNP discovery and development of an interspecific cotton genome map using a simplified genotyping-by-sequencing (GBS) approach

Authors
item Verma, Surender -
item Young, Carla
item Pepper, Alan -
item McCormick, Ryan
item Percy, Richard
item Yu, John

Submitted to: International Cotton Genome Initiative Workshop
Publication Type: Abstract Only
Publication Acceptance Date: September 1, 2012
Publication Date: N/A

Technical Abstract: Recent developments in next-generation sequencing (NGS) technology have lowered the cost of sequencing per base and enabled whole genome re-sequencing, genome-wide association studies, and for some species, unprecedented discovery of molecular markers. For species with large, complex genomes, genotyping-by-sequencing (GBS) of reduced representation libraries has emerged as a promising tool for association studies and genomics-assisted breeding. We used a simplified GBS approach to genotype recombinant inbred lines (RILs) of an interspecific cross between Gossypium hirsutum x G. barbadense (TM-1 x 3-79) to discover novel SNPs and develop an interspecific linkage map. The GBS approach presented here provides a powerful tool for developing informative markers in species without a sequenced genome, while also providing a valuable resource for anchoring physical maps and ordering scaffolds from whole genome sequencing projects. The GBS approach will have broad applications in SNP marker-assisted breeding in taxa with limited prior knowledge of the genome or genetic diversity.

Last Modified: 11/28/2014
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