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United States Department of Agriculture

Agricultural Research Service

Research Project: Intervention Strategies to Control and Prevent Enteric Viral Diseases of Poultry

Location: Endemic Poultry Viral Diseases Research Unit

Title: Reverse genetics of avian metapneumoviruses

Author
item Yu, Qingzhong

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: October 1, 2012
Publication Date: N/A

Technical Abstract: An overview of avian metapneumovirus (aMPV) infection in turkeys and development of a reverse genetics system for aMPV subgroup C (aMPV-C) virus will be presented. By using reverse genetics technology, we generated recombinant aMPV-C viruses containing a different length of glycoprotein (G) gene or a deletion of the M2-2 gene. The biological properties of these recombinant viruses were assessed in Vero cells and in specific-pathogen-free turkeys. The results demonstrated that the large portion of the extracellular domain of the viral attachment protein and the M2-2 gene are not essential for virus viability in cell cultures and in turkeys, but may be required for sufficient virus replication to maintain virus pathogenicity and immunogenicity in a natural host.

Last Modified: 12/20/2014
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