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United States Department of Agriculture

Agricultural Research Service

Research Project: TECHNOLOGIES FOR THE DETECTION OF CHEMICAL AND BIOLOGICAL CONTAMINANTS IN FOODS

Location: Residue Chemistry and Predictive Microbiology

Title: Development of LC-MS/MS method for analysis of polyphenolic compounds in juice, tea and coffee samples

Author
item Sapozhnikova, Yelena

Submitted to: Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: October 26, 2013
Publication Date: November 27, 2013
Citation: Sapozhnikova, Y.V. 2013. Development of LC-MS/MS method for analysis of polyphenolic compounds in juice, tea and coffee samples. Food Chemistry. http://dx.doi.org/10.1016/j.foodchem.2013.10.131.

Interpretive Summary: Polyphenolic compounds are naturally occurring chemicals with a variety of biological activities and numerous potential health benefits. Studies on polyphenols in food samples are important as they generate necessary information on their role and benefits to human health and food quality. This study developed a fast, simple, and cost-effective method for simultaneous determination of multiple polyphenols in juice, tea and coffee samples. The sample preparation was based on a simple “dilute and shoot” approach. The detection was performed using liquid chromatography-tandem mass-spectrometry (LC-MS/MS). The method was successfully applied to the analysis of juice, tea, and coffee samples. The results suggest that the developed method has a promising potential for the analysis of a wide range of polyphenolic compounds. The generated information on occurrence of these chemicals in foods is critical for estimating their content in food, effects on human health and population intake.

Technical Abstract: A simple and fast method for the analysis of a wide range of polyphenolic compounds in juice, tea, and coffee samples was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was based on a simple sample preparation “dilute and shoot” approach, and LC-MS/MS triple quadrupole detection with electrospray ionization and quantification using genistein-d4 as an internal standard. The performance of six different syringeless filter devices was tested for sample preparation. The method was evaluated for recoveries of polyphenols at two spiking levels (250 and 500 ng/mL) in water, juice, tea, and coffee samples. The recoveries of the majority of polyphenols were satisfactory (70-120%), but some varied significantly (35-165%) depending on the matrix. Method detection limits ranged from 1 to 25 ng/mL. NIST Standard Reference Materials (SRM) 3257 Catechin Calibration Solutions and 3255 Camellia sinensis (Green Tea) Extract with certified concentrations of catechin and epicatechin were used for method validation. The measurement accuracy in two SRMs was 72-113%. The method was applied to the analysis of juice, tea, and coffee samples.

Last Modified: 12/21/2014
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