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United States Department of Agriculture

Agricultural Research Service

Research Project: UMBRELLA PROJECT FOR FOOD SAFETY

Location: Warmwater Aquaculture Research Unit

Title: Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages

Authors
item Donaldson, Jr -
item Nanduri, B -
item Pittman, Jr -
item Givaruangsawar, S -
item Burgess, S -
item Lawrence, M -

Submitted to: Journal of Proteomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: May 2, 2011
Publication Date: September 6, 2011
Citation: Donaldson, J.B., Nanduri, B., Pittman, J., Givaruangsawar, S., Burgess, S.C., Lawrence, M.L. 2011. Proteomic expression profiles of virulent and avirulent strains of Listeria monocytogenes isolated from macrophages. Journal of Proteomics. 74(10):1906-1917.

Interpretive Summary: A procedure to determine the survival of L. monocytogenes in macrophages was developed. This can serve to screen L. monocytogenes for this trait.

Technical Abstract: Listeria monocytogenes is able to survive and proliferate within macrophages. In the current study, the ability of three L. monocytogenes strains (serovar 1/2a strain EGDe, serovar 4b strain F2365, and serovar 4a strain HCC23) to proliferate in the murine macrophage cell line J774.1 was analyzed. We found that the avirulent strain HCC23 was able to initiate an infection but could not establish prolonged infection within the macrophages. By contrast, strains EGDe and F2365 proliferated within macrophages for at least 7h. We further analyzed these strains by comparing their protein expression profiles at 0h, 3h, and 5h post-infection using multidimensional protein identification technology coupled with electrospray ionization tandem mass spectrometry. Our results indicated that similar metabolic and cell wall associated proteins were expressed by all three strains at 3h post-infection. However, increased expression of stress response and DNA repair proteins was associated with the ability to proliferate in macrophages at 5h post-infection. By comparing the protein expression patterns of these three L. monocytogenes strains during intracellular growth in macrophages, we were able to detect biological differences that may determine the ability of L. monocytogenes to survive in macrophages.

Last Modified: 9/10/2014
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