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United States Department of Agriculture

Agricultural Research Service

Research Project: INTERVENTIONS TO REDUCE FOODBORNE PATHOGENS IN SWINE AND CATTLE

Location: Food and Feed Safety Research

Title: Rapid detection of Salmonella in bovine lymph nodes using a commercial real-time PCR system

Authors
item Hill, Joshua
item Edrington, Thomas
item Loneragan, Guy -
item Gragg, Sara -
item Nisbet, David

Submitted to: PostDoc Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: January 8, 2013
Publication Date: March 1, 2013
Repository URL: http://handle.nal.usda.gov/10113/57356
Citation: Hill, J.E., Edrington, T.S., Loneragan, G., Gragg, S.E., Nisbet, D.J. 2013. Rapid detection of Salmonella in bovine lymph nodes using a commercial real-time PCR system. PostDoc Journal. 1:35-39.

Interpretive Summary: Cattle may contain the bacteria Salmonella that can make people sick. Food production facilities routinely test their products for contamination. This report describes development of a rapid method for testing for the bacteria Salmonella. This rapid testing may allow food production facilities a quicker turn around to test their perishable products.

Technical Abstract: Rapid Salmonella detection is needed to help prevent the distribution of contaminated food products. Using traditional culture methods, Salmonella detection can take up to 3-5 days. Using an improved protocol and a commercial real-time PCR system, we have shortened the detection time to under 24 hours with the same sensitivity as compared to traditional Salmonella culture methods.

Last Modified: 10/20/2014
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