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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING ARTHROPODS: INTEGRATED PEST MANAGEMENT

Location: Mosquito and Fly Research Unit

Title: Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth

Authors
item Cuomo, Christina -
item Desjardins, Christopher -
item Bakowski, Malina -
item Goldberg, Jonathan -
item Becnel, James
item Didier, Elizabeth -
item Fan, Lin -
item Heiman, David -
item Levin, Joshua -
item Young, Sarah -
item Zeng, Qiandong -
item Troemel, Emily -

Submitted to: Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 9, 2012
Publication Date: January 30, 2013
Citation: Cuomo, C.A., Desjardins, C.A., Bakowski, M.A., Goldberg, J., Becnel, J.J., Didier, E.S., Fan, L., Heiman, D.I., Levin, J.Z., Young, S., Zeng, Q., Troemel, E.R. 2013. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Genome Research. 22:2478-2488.

Interpretive Summary: Naturally occurring protozoan parasites (Microsporidia) of mosquitoes are under study to evaluate and develop these disease causing organisms as biological control agents. Microsporidian parasites are known to cause mortality in mosquitoes worldwide, but fundamental knowledge on the genomes of mosquito microsporidia is unknown. This genomic investigation conducts an analysis, for the first time, on the genome of the mosquito microsporidian Vavraia culicis. The new information obtained here contributes to our basic understanding of the organization of the genomes of these parasites which will assist in the evaluation and development of microsporidia as biocontrol agents.

Technical Abstract: Microsporidia comprise a large phylum of obligate intracellular eukaryotes that are fungalrelated parasites responsible for widespread disease, and here we address questions about microsporidia biology and evolution. We sequenced three microsporidian genomes from two species, Nematocida parisii and Nematocida sp1, which are natural pathogens of Caenorhabditis nematodes and provide model systems for studying microsporidian pathogenesis. We performed deep-sequencing of transcripts from a timecourse of N. parisii infection. Examination of pathogen gene expression revealed compact transcripts and a dramatic takeover of host cells by Nematocida. We performed phylogenomic analyses of Nematocida and other microsporidian genomes to refine microsporidian phylogeny and identify evolutionary events of gene loss, acquisition, and modification. In particular, we found that all microsporidia lost the tumor suppressor gene Retinoblastoma, which we speculate could accelerate the parasite cell cycle and increase mutation rate. We also found that microsporidia acquired transporters that could import nucleosides to fuel rapid growth. In addition microsporidian hexokinases gained secretion signal sequences, and in a functional assay these were sufficient to export proteins out of the cell; thus hexokinase may be targeted into the host cell to reprogram it toward biosynthesis. Similar molecular changes appear during formation of cancer cells and may be evolutionary strategies adopted independently by microsporidia to proliferate rapidly within host cells. Finally, analysis of genome polymorphisms revealed evidence for a sexual cycle that may provide genetic diversity to alleviate problems caused by clonal growth. Together these events may explain the emergence and success of these diverse intracellular parasites.

Last Modified: 10/19/2014
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