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United States Department of Agriculture

Agricultural Research Service

Research Project: DEVELOP METHODS TO ASSESS AND IMPROVE POULTRY AND EGG QUALITY

Location: Quality and Safety Assessment Research Unit

Title: Lc-Ms and Gc-Tofms Analysis of Maclura Pomifera Extracts

Author
item Holser, Ronald

Submitted to: Montreux Symposium on Liquid Chromatography/Mass Spectrometry
Publication Type: Abstract Only
Publication Acceptance Date: July 4, 2012
Publication Date: November 7, 2012
Citation: Holser, R.A. 2012. LC-MS and GC-TOFMS analysis of maclura pomifera extracts [abstract]. Montreux Symposium on Liquid Chromatography/Mass Spectrometry. 2012 CDROM.

Interpretive Summary: The rapid analysis of plant extracts for bioactive compounds was performed using a combination of UHPLC-MS and GC-TOFMS. Both techniques generate mass spectra that are useful for the determination of natural product structures. Alcohol extracts were obtained from Maclura pomifera (osage orange), a species known to contain numerous bioactive compounds, and analyzed with an Accela UHPLC-MSQ-PDA system (ThermoFisher Scientific, Waltham, Massachusetts, USA). Separations were performed using a C18 column. The MS detector was operated in negative mode with the electrospray interface. The diode array detector scanned between 200 and 400 nm. Samples were also analyzed by a Leco GC equipped with the Pegasus IV TOF detector (Leco Corp., St. Joseph, Michigan, USA). Spectral data were collected by both instruments and correlated to develop a library for the routine analysis of samples by LC-MS. This approach provided the sensitivity of the TOF system to identify target compounds with the use of LC-MS to quickly screen samples.

Technical Abstract: The rapid analysis of plant extracts for bioactive compounds was performed using a combination of UHPLC-MS and GC-TOFMS. Both techniques generate mass spectra that are useful for the determination of natural product structures. Alcohol extracts were obtained from Maclura pomifera (osage orange), a species known to contain numerous bioactive compounds, and analyzed with an Accela UHPLC-MSQ-PDA system (ThermoFisher Scientific, Waltham, Massachusetts, USA). Separations were performed using a C18 column. The MS detector was operated in negative mode with the electrospray interface. The diode array detector scanned between 200 and 400 nm. Samples were also analyzed by a Leco GC equipped with the Pegasus IV TOF detector (Leco Corp., St. Joseph, Michigan, USA). Spectral data were collected by both instruments and correlated to develop a library for the routine analysis of samples by LC-MS. This approach provided the sensitivity of the TOF system to identify target compounds with the use of LC-MS to quickly screen samples.

Last Modified: 4/19/2014
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