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United States Department of Agriculture

Agricultural Research Service

Research Project: FUNCTIONAL GENOMICS FOR IMPROVING NUTRIENTS AND QUALITY IN ALFALFA AND SOYBEAN

Location: Plant Science Research

Title: How resilient is the soybean genome? Insights from fast neutron mutagenesis

Authors
item Bolon, Yung-Tsi -
item Stec, Adrian -
item Roessler, Jeffrey -
item Michno, Jean-Michel -
item Anderson, Justin -
item Muehlbauer, Gary -
item Vance, Carroll
item Stupar, Robert -

Submitted to: Biennial Conference on Molecular and Cellular Biology of the Soybean
Publication Type: Abstract Only
Publication Acceptance Date: August 11, 2012
Publication Date: August 13, 2012
Citation: Bolon, Y., Stec, A., Roessler, J., Michno, J., Anderson, J., Muehlbauer, G., Vance, C.P., Stupar, R. 2012. How resilient is the soybean genome? Insights from fast neutron mutagenesis [abstract]. Molecular & Cellular Biology of the Soybean Conference, August 12-15, 2012, Des Moines, Iowa. Available: http://www.extension.iastate.edu/registration/events/soybean/speakeranchor.htm#speaker26.

Technical Abstract: Previously, we described the development of a fast neutron mutant population resource in soybean and identified mutations of interest through phenotypic screening. Here, we consider the resiliency of the soybean genome by examining genomic rearrangements and mutations that arise from fast neutron radiation damage and repair. We previously documented deletions and duplications in a limited number of lines by comparative genomic hybridization and exome resequencing. Approximately two copy number variation events that ranged from one kilobase to three megabases in size were discovered per mutant line. Next-generation sequencing data on the genomic DNA of select mutant lines and data on more than a hundred comparative genomic hybridizations have now been generated. Parallel studies were performed on a subset of mutant lines that showed no visible phenotype. Analyses of these datasets enable characterization of translocations, inversions, insertions, and point mutations in addition to deletions and duplications sustained in the soybean genome. These studies locate fragile or adjustable sites on soybean chromosomes, provide insight on gene essentiality and function, and contribute to the demarcation of a minimum soybean genome.

Last Modified: 4/18/2014
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