ARS | Publication request: Serological examination of sera from hyperimmunized chickens and turkeys

Submitted to: International Symposium on Avian Influenza
Publication Type: Abstract Only
Publication Acceptance Date: June 15, 2012
Publication Date: August 4, 2012
Citation: Pedersen, J.C., Kapczynski, D.R., Suarez, D.L., Hines, N., Killian, M., Schmitt, B.J. 2012. Serological examination of sera from hyperimmunized chickens and turkeys [abstract]. International Symposium on Avian Influenza. CDROM.

Technical Abstract: Detection of avian influenza (AI) H5 and H7 antibody in commercial poultry is a World Organization for Animal Health (OIE) notifiable event and impacts trade. Chickens and turkeys which had been vaccinated with an oil-emulsion H1N1/H3N2 influenza autogenous vaccine were inoculated with inactivated pandemic H1N1 (pH1N1) virus (chickens) and live pH1N1 virus (turkeys) to determine if multiple exposures to pH1N1 virus can result in non-specific hemagglutination-inhibition (HI) reactions against H5 AI. Sera from the hyperimmunized chickens and turkeys and field vaccinated turkeys were tested to evaluate the possibility of cross-reaction to subtypes H1, H2, and H5 AI which genetically fall into a distinct phylogenetic clade. Sera from field turkeys and hyperimmunized chickens and turkeys did result in a small number of positive H5 reactions, but the results do not appear to support cross-reactivity based on a clade-based relationship.