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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #279441

Title: Environmental optimization for production of 7, 10-dihydroxy-8(E)-octadecenoic acid from olive oil by Pseudomonas aeruginosa PR3

Author
item SOHN, HYE-RAN - Kyungpook National University
item Hou, Ching
item KIM, HAK-RYUL - Kyungpook National University

Submitted to: Journal of Biotechnology and Bioprocess Engineering
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2013
Publication Date: 12/13/2013
Citation: Sohn, H., Hou, C.T., Kim, H. 2013. Environmental optimization for production of 7, 10-dihydroxy-8(E)-octadecenoic acid from olive oil by Pseudomonas aeruginosa PR3. Journal of Biotechnology and Bioprocess Engineering. 2:352-356.

Interpretive Summary: A microbial culture called PR3 produces hydroxy fatty acids from linoleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals including special military nylon, plasticizers, coating materials, and possible as physiologically active agents. However, an economical process, ideally direct from natural oils and not from expensive free fatty acids, is needed to produce these value-added hydroxyl fatty acid products. In this research we developed the optimized environmental conditions for efficient production of DOD (dihydroxy fatty acid) from olive oil by PR3 with an 85% conversion yield over the oleic acid content in olive oil. Our findings can facilitate the production of hydroxy fatty acids from olive oil by bioprocess and benefit other researchers.

Technical Abstract: Microbial conversions of free unsaturated fatty acids often generate novel hydroxy fatty acids (HFA), which are known to have special properties such as higher viscosity and reactivity. Among microbial strains known to produce HFAs, Pseudomonas aeruginosa PR3 has been well studied to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from free oleic acid. Previously we reported that strain PR3 could utilize olive oil instead of oleic acid as a substrate for production of DOD (Appl Microbiol Biotechnol 2011, 89:1721-1727). In this study, we developed the optimized environmental conditions for DOD production from olive oil by PR3. DOD production was closely related to the species and concentration of carbon source. Glucose and galactose showed optimal DOD production at 0.2% and 0.4%, respectively. Addition of 1.5% oil substrate to the culture beyond 36 hrs after initiation of cultivation under 200 rpm represented a maximal 318 mg DOD production in 50 ml culture, corresponding to 85% conversion yield over the oleic acid content in olive oil.