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United States Department of Agriculture

Agricultural Research Service

Research Project: EPIDEMIOLOGY AND MANAGEMENT OF XYLELLA FASTIDIOSA (XF) AND OTHER EXOTIC AND INVASIVE DISEASES AND INSECT PESTS Title: Physiological and proteomic characterizations of “Candidatus Liberibacter” associated diseases in citrus and potato plants

Authors
item Nwugo, Chika
item Lin, Hong
item Duan, Ping

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 4, 2012
Publication Date: August 3, 2012
Citation: Nwugo, C.C., Lin, H., Duan, Y. 2012. Physiological and proteomic characterizations of “Candidatus Liberibacter” associated diseases in citrus and potato plants. Phytopathology. 102:S4.71.

Technical Abstract: “Candidatus Liberibacter” species (Lib) are fastidious gram-negative bacteria transmitted by psyllids and associated with citrus huanglongbing (HLB) and potato zebra chip (ZC) diseases. Here, proteomic analyses via 2-DE and mass spectrometry were employed to elucidate protein expression profiles in tissues of grapefruit, lemon and potato plants that were infected or uninfected with Lib. Inductively Coupled Plasma Spectroscopy was also used to identify the effect of Lib infection on nutrient status of same plants. The analysis indicated that Lib infection altered protein expression profiles in leaves of grapefruit (200 spots) and lemon (70 spots). Protein profiles of aboveground (107 spots) and belowground (95 spots) tissue of potatoes also were differentially expressed. Interestingly, Matrix-Assisted Laser Desorption/Ionization-Time of Flight and Liquid Chromatography-Mass Spectrometry analysis showed that chitinase, starch synthase and patatin are among a group of proteins that were significantly up-regulated in citrus and/or potato tissues in response to Lib infection. Furthermore, nutrient status analysis suggests that accumulation of Zn and Ca in citrus plants, as well as Fe, Cu, and particularly K, in potato plants is associated with Lib infection. In summary, results provide insights into potential host-specific response mechanisms associated with HLB and ZC.

Last Modified: 12/20/2014
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