ARS | Publication request: “Parts Is Parts,” at least from the proteomics perspective

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: February 21, 2012
Publication Date: March 3, 2012
Citation: Miernyk, J.A. 2012. “Parts Is Parts,” at least from the proteomics perspective. In: Mock, H-P., Wang, Zz-Y, editors. Frontiers in Agriculture Proteome Research.Tsukuba, Japan: Tsukuba Science City Press. p. 24-28.

Technical Abstract: A shot-gun proteomics strategy was used to compare leaves, developing pods, seedcoats, and cotyledons, and suspension-cultured cells of soybean (Glycine max (L.) Merr., cultivar Jack). A total of 3,500 proteins were identified and sorted into 11 categories. Unexpectedly, and with a few notable exceptions, distribution of proteins among the categories was more similar than dissimilar. In all instances the Primary Metabolism cluster was most populated, followed by Cellular Structure. The Stress Response cluster contained the smallest number of identified proteins. We speculate that the major differences in organ size, shape, and function are more reflective of posttranslational modifications than the overall bulk proteome.