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United States Department of Agriculture

Agricultural Research Service

Research Project: NOVEL TECHNOLOGY FOR RENEWABLE RESOURCE UTILIZATION

Location: Renewable Product Technology Research Unit

Title: Optimization of environmental conditions for production of a novel cold-active lipase from Pichia lynferdii Y-7723

Authors
item Park, S -
item Kwon, M -
item Hou, Ching
item Kim, H -

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: May 2, 2012
Publication Date: May 2, 2012
Citation: Park, S., Kwon, M.H., Hou, C.T., Kim, H.R. 2012. Optimization of environmental conditions for production of a novel cold-active lipase from Pichia lynferdii Y-7723 [abstract]. American Oil Chemists' Society. Paper #BIO-8.

Technical Abstract: Lipases with abnormal functionality such as high thermostability and optimal activity at extreme conditions gain special attentions because of their applicability in the restricted reaction conditions. In particular, cold-active lipase(CAL)s have gained special attention in various industrial fields such as laundry detergents, pharmaceutical catalysts, and production of structured lipids. However, the sources of CAL is mostly limited in psychrophilic microorganisms. Previously we have reported about finding a novel cold-active lipase from Pichia lynferdii Y-7723 which is a mesophilic yeast strain (Production of a Novel Cold-Active Lipase from Pichia lynferdii Y-7723, Hak-Ryul Kim et al., J. Agric. Food Chem. 2010, 58, 1322-1326). In this study, we investigated the optimization of fermentation conditions for production of a novel cold-active lipase from P. lynferdii Y-7723. Based on the optimization study, optimal lipase productivity was obtained at 330-360 hour incubation at 20**oC with 2% oil substrate in a medium composed of fructose as a carbon source. Among carbon sources tested, fructose showed almost three times high lipase production as the control medium while cell growth was similar. Yeast extract was the most effective nitrogen source.

Last Modified: 4/17/2014
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