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United States Department of Agriculture

Agricultural Research Service

Research Project: RESPONSE OF DIVERSE RICE GERMPLASM TO BIOTIC AND ABIOTIC STRESSES

Location: Dale Bumpers National Rice Research Center

Title: Identification of expressed genes in the mapped QTLs for yield related traits in rice

Authors
item Venu, R-C -
item Jia, Yulin
item Liu, Guangjie -
item Jia, Melissa
item Nobuta, Kan -
item Sreerekha, M-V -
item Moldenhauer, Karen -
item Pellgrini, Matteo -
item Jacobsen, Steven -
item McClung, Anna
item McClung, Anna

Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: December 3, 2011
Publication Date: February 27, 2012
Citation: Venu, R., Jia, Y., Liu, G., Jia, M.H., Nobuta, K., Sreerekha, M., Moldenhauer, K.A., Pellgrini, M., Jacobsen, S.E., Mcclung, A.M. 2012. Identification of expressed genes in the mapped QTLs for yield related traits in rice. Rice Technical Working Group Meeting Proceedings. Feb. 27 - Mar. 1, 2012. Hot Springs, AR. pg. 61.

Technical Abstract: Improvement of grain yield is a perpetual goal in rice breeding. Yield and its component traits are quantitatively inherited and controlled by many genes. To identify the Quantitative Trait Loci (QTL) involved in yield, a recombinant inbred line (RIL) population consisting of 259 progeny was developed from a cross between japonica cultivar Nipponbare and indica cultivar 93-11 using a single seed descent method. Phenotypic characterization of yield related traits (number of days to heading, lodging, tiller angle, number of tillers, number of days to harvest, panicle length, number of branches in the main panicle, number of kernels per main panicle, thousand grain weight and total yield per plant) in the RILs and parents was performed in field studies conducted at Dale Bumpers National Rice Research Center (DBNRRC), Stuttgart, Arkansas (Years 2009, 2010, 2011) and Rice Research Unit (RRU), Beaumont, Texas (years 2009 and 2010), and in a greenhouse study during 2010-2011 at DBNRRC. Transgressive segregation was observed among the RILs for number of days to heading, number of days to harvest, plant height, number of tillers per plant, number of kernels per main panicle and total grain yield per plant. A total of 259 F5-F7 RILs were genotyped with 131 simple sequence repeat (SSR) markers. Composite interval mapping (CIM) was conducted using Windows QTL Cartographer (version 2.5) to identify QTL affecting each yield-related trait. More than 60 QTL related to yield traits have been mapped. Several novel QTLs related to tiller angle, panicle length, days to heading, number of primary branches per panicle, number grains per panicle and grain yield per panicle have been identified. Using Massively Parallel Signature Sequencing (MPSS) technology, the leaf, root and meristem transcriptomes in the parents (Nipponbare and 93-11) and their F1 hybrids were sequenced. Sequence analyses identified many up-regulated genes in F1 hybrid located in the mapped yield QTL regions. Further characterization of these candidate genes in the QTL regions will lead the development of new DNA markers for rice yield improvement.

Last Modified: 7/22/2014
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