Technical Abstract: Thirty Plant Introductions (PIs) from the USDA-ARS National Plant Germplasm System were evaluated for resistance to Cercospora beticola in an artificially produced epiphytotic at the Saginaw Valley Research and Extension Center near Frankenmuth, MI. Internal controls included a susceptible commercial variety, 'CE', and a resistant variety, 'ACH 355'. The field was planted May 5. The nursery was inoculated once with a liquid spore suspension of Cercospora beticola on July 7. Visual evaluations on the plot, producing a disease index (DI) on a scale from 0 (no disease) to 10 (plant dead), were made on four dates, with the peak of the epidemic occurring around the third date. By the fourth date, regrowth of several PIs was visible, and several PIs were dieing after having produced seed stalks, so ratings started to go down and were not recorded. At the third evaluation, means of the resistant and susceptible internal control were 3.5 and 5.9, respectively, across the nursery. In 2010, these means were 3.2 and 5.5, respectively. Means of the PIs ranged from 3.7 to 6.0. An analysis of variance on the disease indices determined that there were significant differences among entries (P=0.05) on all three dates of evaluation. One accessions was not significantly different from the resistant control at the first and third rating dates. Only six accessions and the two control varieites did not require removal of seed stalks during the course of the ratings.