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United States Department of Agriculture

Agricultural Research Service

Research Project: BITING AND STINGING PESTS: ECOLOGY AND BIOLOGICALLY-BASED CONTROL

Location: Biological Control of Pests Research Unit

Title: Identification of fertiity restores for S male-sterile maize: beyond PPRs

Authors
item Chase, Christine -
item Williams-Carrier, Rosalind -
item Barkan, Alice -
item Feole, Michelle -
item Hannah, L.CURT -
item Zhao, Liming
item Kamps, Terry -
item Gabay-Laughnan, Susan -

Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Proceedings
Publication Acceptance Date: December 4, 2011
Publication Date: January 18, 2012
Repository URL: http://pag.confex.com/pag/xx/webprogram/Paper1813.html
Citation: Chase, C., Williams-Carrier, R., Barkan, A., Feole, M., Hannah, L., Zhao, L., Kamps, T., Gabay-Laughnan, S. 2012. Identification of fertiity restores for S male-sterile maize: beyond PPRs. Annual International Plant & Animal Genome Conference. PAGXX: 1813. http://pag.confex.com/pag/xx/webprogram/Paper1813.html.

Technical Abstract: Nuclear genes are essential for expression of the mitochondrial genome and for the function of mitochondrial protein complexes. Interaction of the plant mitochondrial and nuclear genetic systems is exemplified by mitochondrial-encoded cytoplasmic male sterility (CMS) under the control of nuclear fertility restoration (restorer) genes. Most restorer genes cloned to date encode pentatricopeptide repeat (PPR) proteins that regulate post-transcriptional expression of mitochondrial CMS genes. Restorer PPR genes have evolved specificity for CMS gene silencing, in contrast to the majority of PPR genes with functions central to organelle gene expression. The CMS-S system of maize is characterized by a pollen collapse phenotype, and by numerous restorer mutations that rescue pollen function but condition homozygous-lethal seed phenotypes. A collection of restorer mutants was recovered from Mutator (Mu) transposon-active, CMS-S lines. Profiling mitochondrial proteins and transcripts in normal-cytoplasm and restored, CMS-S pollen associated several non-allelic mutants with post-transcriptional failure to accumulate mitochondrial-encoded respiratory subunits. Illumina sequencing of Mu-flanking regions demonstrated the co-segregation of one restorer mutation with a Mu insertion in a gene encoding the mitochondrial-targeted ribosomal protein L6. Restorer mutations therefore rescue CMS-S pollen by disrupting central features of mitochondrial gene expression that are expendable in pollen but essential for seed development. CMS-S fertility restoration affords a novel genetic screen that can be used to investigate the nuclea

Last Modified: 8/27/2014
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