Technical Abstract: The poisonous nature of botulinum neurotoxin (BoNT) poses a great risk to humans and also can be exploited as a possible bioterrorism and biological warfare agent. BoNT serotypes A and B have emerged as effective treatments for a variety of neurological disorders, in addition to their applicability in growing cosmetic industry. Due to its extreme toxicity and applicability as a drug, there is an urgent need to develop sensitive detection assays and therapeutic counter measures. In the last decade, significant progress has been made in BoNT detection technologies but none are capable to replacing the mouse bioassay, considered the gold standard. Recently, new advances in robotics and availability of new research reagents has allowed development of automated multiplex methods for the quicker toxin analysis. These technologies need further refinement in order to generate an ideal assay for BoNTs.