Author
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Cray, Paula |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 10/3/2011 Publication Date: 10/11/2011 Citation: Cray, P.J. 2011. On farm sampling for salmonella: Impact of method and design on results. Delmarva Poultry Industry, National Meeting on Poultry Health and Processing. October 11-13, 2011. Ocean City, Maryland. Interpretive Summary: Technical Abstract: Current sampling for the National Antimicrobial Resistance Monitoring System (NARMS) is comprised primarily of receipt of Salmonella isolates from the USDA FSIS as part of their regulatory compliance testing. These isolates are received from all commodities and product classes. Isolates are characterized for susceptibility to a panel of antimicrobials important in human and veterinary medicine and are also subjected to Pulsed Field Gel Electrophoresis (PFGE) to determine relatedness between isolates. FSIS has changed their regulatory programs over the years and from 2006 to 2011 sampling has been risk based. Following an FDA Science Board review, the NARMS program has sought to modify the animal arm to include isolates originating on-farm (ideally) or at a minimum from pre-intervention points in plant. Changing the sampling scheme has resulted in discussion with FSIS to explore sampling pre-intervention in plant, with commodity groups including direct contact with company representative, and with USDA APHIS. Pros and cons are associated with sampling on-farm, after transport, from lairage, post-kill, post-chill or at retail and sample site may limit our ability to associate antimicrobial use with isolate collected further from the farm. In April 2011 we began a pilot on-farm sampling study with the poultry industry. Companies receive boxes containing all materials required for sample collection on farm. Personnel are trained to collect samples using a standard methodology and samples are shipped back to the lab the same day they are collected for processing the next morning. All samples are cultured for Salmonella, Campylobacter, E. coli and enterococci using standardized culture methods. Salmonella, Campylobacter and enterococci isolates are characterized for susceptibility to a panel of antimicrobials using broth microdilution and Salmonella, Campylobacter and enterococci are also serotyped. All isolates are subjected to PFGE. Samples are double blinded and aggregate data is reported. Individual data is sent back to the primary point of contact for the industry for dissemination to individual farms. A questionnaire is also used to capture management and antimicrobial use information. The feasibility of long term sampling and representativeness will be assessed and this program may serve as a basis for future NARMS sampling. |
