Technical Abstract: The durum wheat (Triticum turgidum L., 2n = 2x = 28; AABB genomes) alien disomic substitution 1E(1A) line DGE-2 (PI 663216) was developed by the USDA–ARS, Cereal Crops Research Unit, Northern Crop Science Laboratory, Fargo, North Dakota and released in 2011. DGE-2 has 2n = 28 chromosomes, which are comprised of 26 durum chromosomes and a pair of chromosomes, 1E, from a wild relative, diploid wheatgrass (Lophopyrum elongatum (Host) Á. Löve, 2n = 2x = 14; EE genome). The chromosome 1E pair replaces the 1A pair of the durum cultivar ‘Langdon.’ The disomic substitution is meiotically regular forming 14 bivalents, and is fertile. The pedigree of DGE-2 is [DGE-1 (2n = 28 + 2)] PI 645483/Langdon 1D(1A)*5. We crossed our alien addition line DGE-1 with Langdon disomic substitution line 1D(1A) and produced F1 hybrid derivatives with chromosomes 1A, 1D, and 1E in a single dose. We selfed these hybrid derivatives five times, and screened each generation for homoeologous group-1 chromosomes 1A and 1B. At the same time these hybrid derivatives were tested for the presence of 1E and absence of 1D. Using chromosome-specific molecular markers Xwmc333 for identifying chromosome 1A, Xwgm18 for 1B, Xwmc147 for 1D, and Xedm17 for 1E, we identified and isolated a disomic alien substitution line 1E(1A) that was stable and is released as a new genetic stock.