Technical Abstract: The contamination of ready-to-eat (RTE) meat products with Listeria monocytogenes is a major concern for the food industry. For a better understanding of the adaptation and survival ability of L. monocytogenes grown on turkey deli meat, the transcriptome of L. monocytogenes strain F2365 was determined using a microarray. Microarray data were validated using a quantitative real time RT-PCR assay. Based on the microarray data, 39 and 45 genes from L. monocytogenes were transcriptionally up-regulated and down-regulated, respectively. The genes regulated at the transcriptional level were mainly involved in energy metabolism, fatty acid and phospholipid metabolism, biosynthesis of proteins, transport and binding proteins, DNA metabolism, cellular processes, and regulatory functions. There was no significant change on the expression of genes encoding for known virulence factors such as sigB, prfA, inlA, inlB, plcA, plcB, and hly. This study suggests that L. monocytogenes grown on RTE deli meat changes its transcription involved in its metabolic pathways to obtain an energy source or an ability to adapt to environmental change without increasing the expression levels of virulence factors. The global transcriptome profiles provide a better understanding of the growth or adaptation of L. monocytogenes in RTE meat products.