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United States Department of Agriculture

Agricultural Research Service

Research Project: CHARACTERIZATION AND ENHANCEMENT OF PLANT RESISTANCE TO WATER-DEFICIT AND THERMAL STRESSES

Location: Plant Stress and Germplasm Development Research

Title: Transcriptional Profiling in Cotton Associated with Bacillus Subtilis (UFLA285) Induced Biotic-Stress Tolerance

Authors
item Medeiros, Flavio H. V. -
item Souza, Ricardo -
item Medeiros, Fernanda C.L. -
item Zhang, Huiming -
item Wheeler, Terry -
item Payton, Paxton
item Ferro, Henrique -
item Pare, Paul -

Submitted to: Plant and Soil
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 1, 2011
Publication Date: October 1, 2011
Citation: Medeiros, F., Souza, R.M., Medeiros, F., Zhang, H., Wheeler, T., Payton, P.R., Ferro, H.M., Pare, P.W. 2011. Transcriptional Profiling in Cotton Associated with Bacillus Subtilis (UFLA285) Induced Biotic-Stress Tolerance. Plant and Soil. vol.347issue1-2p.327-337.

Interpretive Summary: Abstract Lint yield and quality in cotton is greatly affected by water-deficit stress. The principal aim of this study was to identify cotton genes associated metabolic pathways involved in the water-deficit stress response. Gene expression profiles were developed for leaf and root tissues subjected to slow-onset water deficit under controlled, glasshouse conditions. The stress was characterized by leaf water potential of -23.1 Bars for stressed tissue compared to -8.7 Bars for fully-irrigated control plants and a corresponding decrease in net photosynthesis to approximately 60% of the rates seen in the irrigated controls (30.3 + 4.7 µmol CO2 m-2 s-1 compared to 17.8 + 5.9 µmol CO2 m-2 s-1). Profiling experiments revealed 2079 stress-responsive transcripts, 854 classified as stress-induced and 1157 stress-repressed in root and/or leaf. Interestingly, the majority of stress-responsive transcripts appear to have tissue-specific expression patterns and only 172 genes showed similar patterns of stress responsive expression in both tissues. A variety of putative metabolic and regulatory pathway were identified using MapMan software and the potential targets for candidate gene selection and ectopic expression to alter these pathways and responses are discussed.

Technical Abstract: Abstract Lint yield and quality in cotton is greatly affected by water-deficit stress. The principal aim of this study was to identify cotton genes associated metabolic pathways involved in the water-deficit stress response. Gene expression profiles were developed for leaf and root tissues subjected to slow-onset water deficit under controlled, glasshouse conditions. The stress was characterized by leaf water potential of -23.1 Bars for stressed tissue compared to -8.7 Bars for fully-irrigated control plants and a corresponding decrease in net photosynthesis to approximately 60% of the rates seen in the irrigated controls (30.3 + 4.7 µmol CO2 m-2 s-1 compared to 17.8 + 5.9 µmol CO2 m-2 s-1). Profiling experiments revealed 2079 stress-responsive transcripts, 854 classified as stress-induced and 1157 stress-repressed in root and/or leaf. Interestingly, the majority of stress-responsive transcripts appear to have tissue-specific expression patterns and only 172 genes showed similar patterns of stress responsive expression in both tissues. A variety of putative metabolic and regulatory pathway were identified using MapMan software and the potential targets for candidate gene selection and ectopic expression to alter these pathways and responses are discussed.

Last Modified: 8/27/2014
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