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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPACT OF EARLY DIETARY FACTORS ON CHILD DEVELOPMENT AND HEALTH Title: Blueberries reduce pro-inflammatory cytokine TNF-alpha and IL-6 production in mouse macrophages by inhibiting NF Kappa B activation and the MAPK pathway

Authors
item Xie, Chenghui -
item Kang, Jie -
item Ferguson, Matthew -
item Nagarajan, Shanmugam -
item Badger, Thomas
item Wu, Xianli -

Submitted to: Molecular Nutrition and Food Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: June 28, 2011
Publication Date: October 1, 2011
Citation: Xie, C., Kang, J., Ferguson, M., Nagarajan, S., Badger, T.M., Wu, X. 2011. Blueberries reduce pro-inflammatory cytokine TNF-alpha and IL-6 production in mouse macrophages by inhibiting NF Kappa B activation and the MAPK pathway. Molecular Nutrition and Food Research. 55(10):1587-1591.

Interpretive Summary: Blueberries (BB) have been reported to attenuate atherosclerosis in apoE deficient (ApoE-/-) mice. The aim of this study was to evaluate the effects of BB in reducing pro-inflammatory cytokine production in mouse macrophages. Protein level and mRNA expression of two pro-inflammatory cytokines TNF-alpha and IL-6 were significantly lower in the peritoneal macrophages from mice fed BB without or with LPS or oxLDL stimulation. Furthermore, blueberry components inhibited the phosphorylation of I kappa B, NF-kappa B p65, MAPK p38 and JNK. All of these are important signaling pathways involved in the production of TNF-alpha and IL-6.

Technical Abstract: Blueberries (BB) have been reported to attenuate atherosclerosis in apoE deficient (ApoE-/-) mice. The aim of this study was to evaluate the effects of BB in reducing pro-inflammatory cytokine production in mouse macrophages. ApoE-/- mice were fed AIN-93G diet (CD) or CD formulated to contain 1% freeze-dried BB for 5 weeks. TNF-alpha and IL-6 were lower in serum of BB fed mice and TNF-alpha expression in aorta was down-regulated with BB feeding. Protein level and mRNA expression of TNF-alpha and IL-6 were significantly lower in the peritoneal macrophages from mice fed BB without or with LPS or oxLDL stimulation. RAW264.7 macrophages were treated with polyphenol-enriched extracts made from the sera of rats fed CD (SEC) or CD containing 10% BB (SEB). SEB significantly inhibited LPS-induced mRNA expression and protein levels of TNF-alpha and IL-6. Furthermore, SEB inhibited the phosphorylation of I kappa B, NF- kappa B p65, MAPK p38 and JNK. All of these are important signaling pathways involved in the production of TNF-alpha and IL-6.

Last Modified: 12/18/2014