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Title: Cloning and expression analysis of three secreted protein genes from wheat stripe rust fungus Puccinia striiformis f. sp. tritici

Author
item DONG, YAN-LING - Northwest Agriculture And Forestry University
item YIN, CHUNTAO - Washington State University
item HULBERT, SCOT - Washington State University
item Chen, Xianming
item KANG, ZHEN-SHENG - Northwest Agriculture And Forestry University

Submitted to: World Journal of Microbiology and Biotechnology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/6/2010
Publication Date: 9/18/2010
Citation: Dong, Y., Yin, C., Hulbert, S., Chen, X., Kang, Z. 2010. Cloning and expression analysis of three secreted protein genes from wheat stripe rust fungus Puccinia striiformis f. sp. tritici. World Journal of Microbiology and Biotechnology. 27:1261-1265.

Interpretive Summary: Stripe rust is a major disease of wheat worldwide . The disease is caused by a fungus that infects and reproduces only on green plants of wheat and some grasses. The fungal genes that encode secreted proteins serving as effectors and avirulence genes are thought to be expressed in haustoria, a special fungal structures formed in plant tissue during the infection process. Three putative secreted protein genes from haustoria of the wheat stripe rust fungus were characterized in this study. Their full-length cDNAs were obtained using a molecular cloning technique. Their expression part of genes ranged from 769 to 1,094 base pairs, encoding predicted proteins of 93 to 203 amino acids without significant similarities to any accessions in the GenBank protein database, but with some homologies to predicted proteins in the stem rust fungus. The predicted products of the genes were characterized as secreted proteins had a much higher expression level in infected leaves than in urediniospores and germinated urediniospores. These genes are good candidates for studying genes involved in the wheat-stripe rust interaction.

Technical Abstract: The Puccinia striiformis f. sp. tritici (Pst) fungus is a biotrophic obligate parasite causing stripe rust, a major disease of wheat worldwide. Pst genes that encode secreted proteins serving as effectors and avirulence genes are thought to be expressed in haustoria. Three putative secreted protein genes from Pst haustoria were characterized in this study. Their full-length cDNAs were obtained using the 50 rapid amplification of cDNA ends (RACE). Their transcripts were ranged from 769 to 1,094 base pairs (bp), encoding predicted proteins of 93 to 203 amino acids without significant similarities to any accessions in the GenBank protein database, but with some homologies to predicted proteins in P. graminis, the stem rust pathogen. The predicted products of the genes were characterized as secreted proteins and designated as PstSP2C7, PstSP11L10 and PstSP11P10. PstSP11L10 had a much higher expression level in infected leaves than in urediniospores and germinated urediniospores and therefore should be a good candidate for a gene involved in the wheat-Pst interaction.