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Research Project: GENETIC IMPROVEMENT OF CITRUS

Location: Subtropical Insects and Horticulture Research

Title: Incidence and Severity of Huanglongbing and Candidatus Liberibacter asiaticus Titer among Field-Infected Citrus Cultivars

Authors

Submitted to: HortScience
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: July 26, 2011
Publication Date: N/A

Interpretive Summary: Huanglongbing (HLB) disease was assessed in eight citrus varieties representing diverse fruit types growing in commercial groves in Florida’s Indian River region, an area with high incidence of HLB. Twenty trees of each cultivar were rated for visual HLB symptoms and leaves were collected to assess the level of the bacterium associated with HLB. There was a strong correlation between HLB symptoms and levels of bacteria detected. Although incidence and severity of HLB varied considerably among the groves, differences in HLB among the varieties were found, even when grove differences were excluded in the analyses. ‘Temple’ tangor showed the most consistently low incidence of HLB symptoms and CLas titer; in contrast, ‘Murcott’ tangor and ‘Minneola’ tangelo had the highest incidence of HLB symptoms and highest levels of the HLB bacteria. These results suggest useful resistance to huanglongbing may be found in conventional citrus cultivars and further work is needed to assess this potential and its commercial value.

Technical Abstract: Incidence and severity of Huanglongbing (HLB) disease were assessed in April, 2010 among eight citrus cultivars representing diverse scion types growing in commercial groves in Florida’s Indian River region, an area with high incidence of HLB. Twenty trees of each cultivar were rated for visual HLB symptoms and leaves were collected for quantification of Candidatus Liberibacter asiaticus (CLas), presumptive causal agent of HLB. There was a strong correlation between HLB incidence and CLas titer, whether on a grove basis or a cultivar basis. Although incidence and severity of HLB varied considerably among the groves, differences among the scions were apparent, even when analyses excluded differences between groves. ‘Temple’ tangor showed the most consistently low incidence of HLB symptoms and CLas titer; in contrast, ‘Murcott’ tangor and ‘Minneola’ tangelo had the highest incidence of HLB symptoms and highest CLas titer. These results suggest useful resistance to huanglongbing may be found in conventional scion cultivars and further work is needed to assess this potential and its commercial value.

   

 
Project Team
Bowman, Kim
Niedz, Randall
Stover, Ed
 
Publications
   Publications
 
Related National Programs
  Plant Genetic Resources, Genomics and Genetic Improvement (301)
  Plant Diseases (303)
 
Related Projects
   ANTIMICROBIAL PEPTIDE EVALUATION FOR HUANGLONGBING, CANKER AND PSYLLID RESISTANCE
   EVALUATION OF HLB/ACP RESISTANCE IN PONCIRUS TRIFOLIATA HYBRIDS AND ASSESSMENT OF DNA MARKERS AND POSSIBLE RESISTANCE GENES
   EVALUATION OF MYB1A GENE EXPRESSION IN CITRUS
   DOORYARD FRUIT CROPS FOR CENTRAL FLORIDA AS AN ALTERNATIVE TO CITRUS TO REDUCE REFUGE FOR ASIAN CITRUS PSYLLID
   EXPLORING AVOCADO VARIABILITY FOR LAUREL WILT RESISTANCE AND OTHER FRUIT QUALITY AND HORTICULTURAL TRAITS FOR PRODUCTION IN EAST-CENTRAL FL
   USDA-ARS CITRUS BREEDING TRUST WITH NEW VARIETIES DEVELOPMENT AND MANAGEMENT CORPORATION
   FOUNDER LINES FOR IMPROVED CITRUS BIOTECHNOLOGY
   NFCA BETWEEN USDA-ARS AND UNIVERSITY OF FLORIDA, IFAS AND FAES
   DEVELOPMENT OF PROMISING NEW SCIONS FOR FLORIDA CITRUS: EXPLOITING HUANGLONGBING (HLB) RESISTANCE AND TOLERANCE
   DEVELOPMENT OF PROMISING SUPERSOUR AND OTHER ROOTSTOCKS RESISTANT TO HUANGLONGBING
   HIGH-THROUGHPUT SCREENING OF TRANSGENIC CITRUS FOR HUANGLUNGBING (HLB) RESISTANCE
   PRODUCTION OF TRANSGENIC COMMERCIAL SCION CULTIVARS RESISTANT TO HLB AND CANKER: CONTINUED AMP APPROACHES AND NOVEL TRANSGENIC STRATEGIES
   ANALYZING LIBERIBACTER ISOLATES UNDETECTABLE BY STANDARD DIAGNOSTIC METHODS IN FLORIDA
   Identification and characterization of HLB-induced small RNAs and mRNAs-towards the understanding of natural defense mechanisms against HLB
   INVESTIGATING MECHANISMS OF PATHOGENESIS AND NATURAL DEFENSE RESPONSES OF CITRUS GREENING (HLB) BY CHARACTERIZING HOST SMALL RNAS AND MRNAS
   SECURE SITE FOR TESTING TRANSGENIC AND CONVENTIONAL CITRUS FOR HLB AND PSYLLID RESISTANCE
   HLB RESISTANCE THROGUH TRANSGENIC EXPRESSION OF SHORT CHAIN FRAGMENT VARIABLE ANTIBODIES AGAINST KEY LIBERIBACTER EPITOPES
 
 
Last Modified: 06/19/2013
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