Technical Abstract: In this work we evaluate a collection of 88 carrot cultivars and landraces for polymorphisms at SSR loci and use the obtained markers to assess the genetic diversity, and we show molecular evidence for divergence between Asiatic and Western carrot genetic pools. The use of primer pairs flanking repetitive motifs allowed successful amplification and separation of products for 30 SSR loci. For each accession, a distinct band pattern was obtained indicating there were no duplicates in the collection. In total, 227 alleles were identified and the number of alleles ranged from 3 to 15 depending on the locus with the mean of 7.6 The results of the Bayesian clustering indicate that the set of accessions used in this study is structured with at least two distinct genetic groups containing 17 and 61 accessions, and for further assessment of genetic diversity the remaining 10 unclassified accessions originating from various world regions were not taken into account. The results of both PCoA and Bayesian clustering are highly congruent and revealed that most of carrot cultivars can be separated into two genetic pools, although there was no clear delimitation between them. The first pool comprised predominantly the landraces originating from continental Asia i.e., Turkey, Pakistan, India, China, and Russia, all of yellow, red, and purple root color, and additionally, two cultivars from Japan, the only two Japanese with red and yellow roots In contrast, cluster 2 comprised mainly European accessions, and additionally seven Japanese, four American, and one from Australia. In this group, only a single breeding material created in German research institute was originally of Asiatic origin. Most of the accessions (92%) developed orange roots typical for western carrot type. Thus, this cluster can be considered as the Western gene pool.