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ARS Home » Midwest Area » Madison, Wisconsin » Vegetable Crops Research » Research » Publications at this Location » Publication #268097
Title: Fine mapping of paternal sorting of mitochondria (psm) in cucumber

Author
item PARK, YOUNG - Pusan National University
item CALDERON, CLAUDIA - Rafael Landivar University
item AL-FAIFI, SULIEMAN - King Saud University
item Havey, Michael

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2011
Publication Date: 11/5/2011
Citation: Park, Y.H., Calderon, C., Al-Faifi, S., Havey, M.J. 2011. Fine mapping of paternal sorting of mitochondria (psm) in cucumber [abstract]. 8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference. Paper No. OP9-1.

Interpretive Summary:

Technical Abstract: Cucumber is unique among plants because its mitochondrial DNA shows paternal transmission, is one of the largest known among all plants, due largely to short repetitive DNA motifs, and undergoes recombination among repeats to produce rearranged mitochondrial DNAs associated with strongly mosaic (MSC) phenotypes. We undertook a germplasm screen to identify cucumber accessions which produced relatively large frequencies of wild-type progenies when crossed with MSC plants as the male. We identified a unique nuclear locus (Psm) which controls sorting of wild-type versus MSC mitochondria transmitted by the male gametophyte. A large segregating family was produced and F2 plants were crossed as the female with MSC16. Over 450 testcross families were scored for numbers of wild-type versus MSC progenies and molecular markers, assigning Psm to a 2.7 cM region on chromosome 3. Eventual cloning of Psm should provide unique insights about nuclear-mitochondrial interactions that control the prevalence of specific mitochondrial DNAs.