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United States Department of Agriculture

Agricultural Research Service

Research Project: IMPACT OF EARLY DIETARY FACTORS ON CHILD DEVELOPMENT AND HEALTH Title: Analysis of polar lipids in the serum from rats fed shiitake by liquid chromatography-mass spectrometry/sass spectrometry

Authors
item Yu, Shanggong -
item Peng, Min -
item Ronis, Martin -
item Badger, Thomas
item Fang, Nianbai -

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 10, 2010
Publication Date: December 22, 2010
Citation: Yu, S., Peng, M., Ronis, M.J., Badger, T.M., Fang, N. 2010. Analysis of polar lipids in the serum from fats fed shiitake by liquid chromatography-mass spectrometry/mass spectrometry. Journal of Agricultural and Food Chemistry. 58(24):12650-12656.

Interpretive Summary: Mushrooms are a food source with potential health benefits, which go above and beyond their nutrient levels. Shiitake mushrooms have been used in cancer treatment and we have studied them for cardiovascular effects. We have found that when animals consume relatively high levels of Shiitake, the blood lipid (fat) profiles are healthier than animals not fed the mushrooms. In this study, we attempted to identify the types of lipids in the mushrooms and in the blood of animals that ate the mushrooms. Our next steps will be to identify which mushroom factors are responsible for the health benefits.

Technical Abstract: Consumption of a shiitake mushroom diet has been reported to have effects on serum phospholipids. However, much less is known about the effect on serum polar lipids, including lysophospholipids and free fatty acids. In the present study, the effects of a shiitake diet were evaluated on the basis of identification and quantification, of individual polar lipid components in rat serum using liquid chromatography-mass spectrometry/mass spectrometry. By comparison with standards and published data, 50 lysophospholipids and 32 free fatty acids were identified, and the concentrations of 27 polar lipids in rat serum were determined. Shiitake diets decreased the levels of all individual polar lipid components in the serum of male rat. The total level of serum polar lipids in males fed 4% shiitake diets (1365.71 mol/L), was significantly lower than that of the control (2270.26 mol/L). However, shiitake diets did not significantly affect the levels of serum polar lipids in female rats.

Last Modified: 11/27/2014