ARS | Publication request: Cloning and transcription profiling of trypsin in Aedes taeniorhynchus (Diptera: Culicdae)

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: February 23, 2011
Publication Date: N/A

Technical Abstract: The cDNA of a trypsin gene from Aedes (Ochlerotatus) taeniorhynchus (Weidemann) was cloned and sequenced. THe full-length mRNA sequence (874 bp_ for trypsin from Ae.taeniorhynchus (AetTryp_ was obtained which encodes an open reading frame of 717 bp (i.e., 239 aa). To detect whether AetTryp is developmentally regulated, a quantitative real-time polymerase chain reaction (qPCR) was used to examin AetTryp mRNA expression levels in different developmental stages of Ae. taeniorhynchus. AetTryp was expressed at low levels in egg, larval, and pupal stages but was differentially expressed in adult Ae. taeniorhynchus, with highest levels found in 5-d-old female adults when compared to teneral adults. In addition, AetTryp mRNA expression differed between sexes, with expression levles much lower in males. However, in both males and females there was a significant increase in AetTryp transcription levels as age increased and peaked in 5-d-old adults. The mRNA transcription levels of AetTryp mRNA expression in response to a blood meal and permethrin treatment in female Ae. taeniorhynchus was determined.