SYSTEMATICS OF MICROFUNGI USED IN BIOLOGICAL CONTROL OF PLANT DISEASES AND INSECT PESTS
Title: Species diversity of Trichoderma in Poland
| Popiel, D. - |
| Blaszczyk, L. - |
| Koczyk, G. - |
| Chelkowski, J. - |
| Samuels, Gary |
Submitted to: Journal of Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 5, 2011
Publication Date: May 10, 2011
Citation: Popiel, D., Blaszczyk, L., Koczyk, G., Chelkowski, J., Samuels, G.J. 2011. Species diversity of Trichoderma in Poland. Journal of Applied Genetics. 52:233-243.
Interpretive Summary: Fungi are a diverse group of organisms that can serve humanity in both good and bad ways. Some fungi are used to control diseases of plants while others are used to produce industrial enzymes. Many fungi have yet to be discovered especially those that occur in soils. In this research the number and kinds of a group of fungi were explored in soils of Central Europe. It was determined that in Central Europe the number of species was low when compared to those in tropical regions. One species that causes a disease of commercial mushrooms was found in mushroom houses but not elsewhere. This report contributes to understanding the worldwide distribution of these fungi and will be used by plant pathologist and biotechnologists using these fungi to control plants and produce industrial enzymes.
Fifteen species of Trichoderma were identified from among 118 strains originating from different regions and ecological niches in Poland. This low number indicates low species diversity of Trichoderma in this Central European region. Using the ITS1-ITS2 regions, 64 strains were positively identified as Trichoderma atroviride (4 strains), T. aggressivum (5), T. citrinoviride (11), T. hamatum (7), T. harzianum (22), T. asperellum (1), T. virens (7), or T. viride (7). When ITS1-ITS2 did not provide unambiguous identification, tef1 was sequenced. This method was applied to an additional fifty-four strains with the following identifications: T. atroviride (12), T. koningi(17), T. viridescens (12), T. koningiopsis (3), T. viride (5), T. polysporum (1), T. gamsii (1), T. tomentosum (1) and T. longibrachiatum (2).