ARS | Publication request: Immunoenhancing effects of MontanideTM ISA oil-based adjuvants on recombinant coccidia antigen vaccination against Eimeria acervulina infection

Submitted to: BARC Poster Day
Publication Type: Abstract Only
Publication Acceptance Date: April 7, 2011
Publication Date: April 18, 2011
Citation: Jang, S.I., Lillehoj, H.S., Lee, S.H., Lee, K., Park, M., Bauchan, G.R., Lillehoj, E., Bertrand, F., Dupuis, L., Deville, S. 2011. Immunoenhancing effects of MontanideTM ISA oil-based adjuvants on recombinant coccidia antigen vaccination against Eimeria acervulina infection. BARC Poster Day. P.20.

Technical Abstract: The current study was conducted to investigate the immunoenhancing effects of Montanide' adjuvants on protein subunit vaccination against avian coccidiosis. Broiler chickens were immunized subcutaneously with a purified Eimeria acervulina recombinant profilin protein, either alone or mixed with one of four adjuvants (ISA 70 VG, ISA 71 VG, ISA 201 VG or ISA 206 VG). Following an oral challenge infection with live E. acervulina oocysts, body weight gains, fecal oocyst shedding, and humoral and innate immune responses were evaluated. Immunization with profilin plus ISA 70 VG or ISA 71 VG increased body weight gains compared with vaccination with profilin alone. Profilin plus ISA 71 VG also reduced fecal oocyst shedding compared with vaccination in the absence of adjuvant. Profilin serum antibody titers were enhanced with all adjuvants. Increased levels of gene transcripts encoding IL-2, IL-10, IL-17A, and IFN-', but decreased levels of IL-15 mRNAs, were observed in intestinal intraepithelial lymphocytes of chickens immunized with profilin plus adjuvants compared with immunization with profilin alone. Finally, increased infiltration of lymphocytes, especially CD8+ lymphocytes at the site of immunization, was observed in broilers given profilin plus ISA 71 VG compared with profilin alone. These results demonstrate that vaccination with the E. acervulina profilin subunit vaccine in combination with MontanideTM adjuvants enhances protective immunity against avian coccidiosis.