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United States Department of Agriculture

Agricultural Research Service

Research Project: HIGHER DIPTERA PESTS OF LIVESTOCK AND POULTRY: SCREWWORM FLIES

Location: Screwworm Research

Title: Genetic variation between populations of the stable fly, Stomoxys calcitrans (L.)(Diptera: Muscidae) from Nebraska, Denmark and Australia

Authors
item Kneeland, Kate -
item SKODA, STEVEN
item Foster, John -

Submitted to: Entomology Society America Annual Meeting
Publication Type: Other
Publication Acceptance Date: February 27, 2011
Publication Date: N/A

Interpretive Summary: The stable fly, Stomoxys calcitrans L., is a cosmopolitan, major pest of livestock. Previous studies on this insect, from samples within the United States, suggested a large amount of gene flow; more genetic variation was detected within populations than between populations. To compare the genetic variability of stable flies on a wider scale, molecular markers generated using Amplified Fragment Length Polymorphisms on samples obtained from Nebraska, Denmark and Australia were analyzed using the Analysis of Molecular Variance. In the populations studied, the greatest genetic variation occurred within populations (80.25%), with only 19.75% between populations. The FST (fixation index) was low at 0.19745 and the Nm (estimate of gene flow) was high at 2.7954. These combined data suggest that there is gene flow occurring in stable flies over long distances. This information is important when developing pest management strategies or in tracking or combating insecticide resistance.

Technical Abstract: The stable fly, Stomoxys calcitrans L., is a cosmopolitan, major pest of livestock. Previous studies on this insect, from samples within the United States, suggested a large amount of gene flow; more genetic variation was detected within populations than between populations. To compare the genetic variability of stable flies on a wider scale, molecular markers generated using Amplified Fragment Length Polymorphisms on samples obtained from Nebraska, Denmark and Australia were analyzed using the Analysis of Molecular Variance. In the populations studied, the greatest genetic variation occurred within populations (80.25%), with only 19.75% between populations. The FST (fixation index) was low at 0.19745 and the Nm (estimate of gene flow) was high at 2.7954. These combined data suggest that there is gene flow occurring in stable flies over long distances. This information is important when developing pest management strategies or in tracking or combating insecticide resistance.

Last Modified: 8/27/2014
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