ARS | Publication request: Proteomic analysis of grapevines in response to Xylella fastidiosa infection

Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: May 20, 2011
Publication Date: June 1, 2011
Citation: Lin, H., Yang, L., Civerolo, E.L., Walker, A.M. 2011. Proteomic analysis of grapevines in response to Xylella fastidiosa infection. American Phytopathological Society Abstracts. 101:S105.

Technical Abstract: Xylella fastidiosa (Xf) is the bacterial causal agent of Pierce’s disease (PD) of grapevines, as well as of other economically important diseases in a number of agronomic, horticultural and ornamental plants. The objective of this research was to tentatively identify proteins that are expressed in grapevines and involved in disease development or defense responses to Xf infection. Comparative analyses were carried out to identify proteins differentially expressed in Xf-infected grape stems from a pair of siblings of 9621-67 (highly susceptible) and 9621-94 (highly resistant) from a cross of Vitis rupestris x V. arizonica. Total proteins were isolated from the stems of uninoculated and Xf-inoculated plants at 1, 6, and 12 weeks after inoculation, separated by a 2D-PAGE system, and spots representing differentially expressed proteins were analyzed and identified using LC/MS/MS. Results revealed that differential expression of proteins in response to Xf-infection were genotype and development stage dependent. This study provides the first proteomic analyses of host responses to Xf infection in highly resistant and susceptible genotypes. The information obtained will aid in the understanding of host-pathogen interactions involved in PD.