|Lee, Ae Kyung -|
|Joung, Young Hee -|
|Wu, Xue Wei -|
|Jung, Heoy Kyung -|
|Burk, John -|
|Sanders, Laurie -|
|Suh, Jeung Keun -|
Submitted to: Scientia Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: April 30, 2011
Publication Date: July 27, 2011
Citation: Lee, A., Joung, Y., Wu, X., Jung, H., Burk, J., Sanders, L., Roh, M.S., Suh, J. 2011. Confirmation of hybrid origin in Arisaema (Araceae) using molecular markers. Scientia Horticulturae. 129:812-827. Interpretive Summary: In general, hybrids are recognized by their morphological intermediacy. The jack-in-the pulpit, Arisaema (Araceae), has typical aroid floral morphology characterized by a fleshy spadix surrounded by a leaf-like spathe. A naturally occurring population of putative hybrids based on the morphological characters between A. triphyllum Schott subsp. stewardsonii and A. dracontium was reported. In one recent study, allozyme analyses suggested that A. ehimenes may be a hybrid between A. serratum and A. tosaense. However, no taxon-specific markers were identified to document the hybrid origin. There is no previous confirmation of the hybrid origin of a species or of putative hybrids of Arisaema using molecular markers. The objectives of this research are to investigate putative A. triphyllum × A. dracontium hybrids using molecular markers. Dendrograms constructed with a combined sequence analysis of A. triphyllum 26S ribosomal RNA gene (SI 467) plus of A. tortuosum phytochrome C-like (phyC) gene (SI 468) were very similar to that of all 19 regions/genes examined. This suggests that selecting SI 467 and SI 468 would be practical to identify hybrid origins involving two parental species.
Technical Abstract: A population of hybrids between Arisaema triphyllum subsp. stewardsonii and A. dracontium was investigated using molecular markers to document the hybrid origin. Total genomic DNA was extracted from A. triphyllum, A. dracontium, and the hybrids, and subjected to sequence analysis of various regions of intergenic spacer and genes of chloroplast or intergenic spacer and genes of nuclear ribosome for single nucleotide polymorphisms (SNPs). Clustering was performed using the unweighted pair group method with averages (UPGMA) tested by bootstrap (BS). Hybrid origin could not be easily confirmed in some regions. However, dendrograms constructed with a combined sequence analysis of A. triphyllum 26S ribosomal RNA gene [sequence identification (SI) 467] plus A. tortuosum phytochrome C-like (phyC) gene (SI 468) were very similar to dendrograms constructed from sequences of all regions. This suggests that selecting SI 467 and SI 468 would be practical to identify hybrid origins involving two parental species. Clustering of hybrids together with the female parent in most target regions suggests that, in Arisaema, cpDNA is considered maternally inherited.