Title: Production of a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid from high oleic safflower oil by Pseudomonas aeruginosa PR3 Authors
|Bae, Jeo-Han -|
|Suh, Min-Jeong -|
|Lee, Na-Young -|
|Kim, Hak-Ryul -|
Submitted to: Journal of Biotechnology and Bioprocess Engineering
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: November 30, 2010
Publication Date: January 30, 2011
Citation: Bae, J., Suh, M., Lee, N., Hou, C.T., Kim, H. 2011. Production of a value-added hydroxy fatty acid, 7,10-dihydroxy-8(E)-octadecenoic acid from high oleic safflower oil by Pseudomonas aeruginosa PR3. Journal of Biotechnology and Bioprocess Engineering. 15:953-958. Interpretive Summary: Previously, we discovered a new microbial culture Pseudomonas aeruginosa PR3 that produced 7,10-dihydroxy-fatty acid from oleic acid and trihydroxy fatty acids from linoleic acid. Hydroxy fatty acids are useful as starting materials for the synthesis of specialty chemicals, special military nylon, plastisizers, coating materials, and possible as physiologically active agents. However, we need an economical process to produce these value-added hydroxyl fatty acid products ideally direct from vegetable oils and not from expensive free fatty acids. Earlier we reported that triolein, the triacylglyceride form of oleic acid, was successfully converted to dihydroxy fatty acids product (DOD) by strain PR3 via triolein-induced lipase activity. Now we found that strain PR3 can also efficiently convert safflower oil directly to produce DOD. Our findings can facilitate the research on hydroxyl fatty acids production from other vegetable oils such as soybean oil by bioprocess and benefit the U.S. farmers.
Technical Abstract: Hydroxy fatty acids (HFA), originally found in small amount mainly from plant systems, are good examples of the structurally modified lipids, rendering special properties such as higher viscosity and reactivity compared to normal fatty acids. Based on these properties, HFAs possess high industrial potentials in a wide range of applications including resins, waxes, nylons, plastics, lubricants, cosmetics, and additives in coatings and paintings. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids since HFA production was limited in plant system. Among microbial strains tested, Pseudomonas aeruginosa PR3 are well studied to produce 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid. Previously we reported that strain PR3 could utilize triolein rather than oleic acid as a substrate for the production of DOD (Appl Microbiol Biotechnol 2007, 74:301-306). In this study, we focused on utilization of vegetable oil as a substrate for DOD production by PR3. Consequently strain PR3 could utilize efficiently the high oleic safflower oil as a substrate for DOD production. Optimal initial medium pH and incubation time were pH 8.0 and 72 hr, respectively. Optimal carbon and nitrogen sources were fructose and glutamine, respectively. Results from this study demonstrated that normal vegetable oils could be used as efficient substrates for the production of value-added hydroxyl fatty acids by microbial bioconversion.